Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- PA5-54282 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZMYND8 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: TTKTDKTSTT GSILNLNLDR SKAEMDLKEL SESVQQQSTP VPLISPKRQI RSRFQLNLDK TIESCKAQLG INEISEDVYT AVEHSDSEDS EKSDSSDSEY ISDDEQKSKN Highest antigen sequence identity to the following orthologs: Mouse - 92%, Rat - 92%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RACK7 in mouse cell line NIH-3T3 and rat cell line NBT-II using a RACK7 Polyclonal Antibody (Product # PA5-54282).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RACK7 in human cell lines Caco-2 and HeLa using a RACK7 Polyclonal Antibody (Product # PA5-54282). Corresponding ZMYND8 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of RACK7 in human cell line A-431 shows positivity in the Golgi apparatus & nucleus but excluded from the nucleoli. Samples were probed using a RACK7 Polyclonal Antibody (Product # PA5-54282).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RACK7 in human cerebral cortex using RACK7 Polyclonal Antibody (Product # PA5-54282) shows strong positivity in neurons.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RACK7 in human liver using RACK7 Polyclonal Antibody (Product # PA5-54282) shows no positivity in hepatocytes as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RACK7 in human cerebellum using RACK7 Polyclonal Antibody (Product # PA5-54282) shows moderate positivity in cells in molecular layer.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RACK7 in human fallopian tube using RACK7 Polyclonal Antibody (Product # PA5-54282) shows moderate nuclear positivity in glandular cells.