H00004857-M12

antibody from Abnova Corporation

Targeting: NOVA1 NOVA-1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00004857-M12

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00004857-M12, RRID:AB_1678979

Product name

NOVA1 monoclonal antibody (M12), clone 3F7

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant NOVA1.

Antigen sequence

MMAAAPIQQNGTHTGVPIDLDPPDSRKRPLEAPPE
AGSTKRTNTGEDGQYFLKVLIPSYAAGSIIGKGGQ
TIVQLQKETGATIKLSKSKDFYPGTTERVCLIQGT
VEALNAVHGFIAEKIREMPQNVAKTEPVSILQPQT
TVNPDRIKQTLPSSPTTTKSSPSDPMTTSRANQVK
IIVPNSTAGLIIGKGGATVKAVMEQSGAWVQLSQK
PDGINLQERVVTVSGEPEQNRKAVELIIQKIQEDP
QSGSCLNISYANVTGPVANSNPTGSPYANTAEVLP
TAAAAAGLLGHANLAGVAAFPAVLSGFTGNDLVAI
TSALNTLASYGYNLNTLGLGLSQAAATGALAAAAA
SANPAAAAANLLATYASEASASGSTAGGTAGTFAL
GSLAAATAATNGYFGAASPLAASAILGTEKSTDGS
KDVVEIAVPENLVGAILGKGGKTLVEYQELTGARI
QISKKGEFVPGTRNRKVTITGTPAATQAAQYLITQ
RITYEQGVRAANPQKVG


Isotype

IgG

Antibody clone number

3F7

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of NOVA1 expression in transfected 293T cell line by NOVA1 monoclonal antibody (M12), clone 3F7.Lane 1: NOVA1 transfected lysate (Predicted MW: 51.7 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged NOVA1 is 1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol