Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- NBP1-00221 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-00221, RRID:AB_1503796
- Product name
- Goat Polyclonal Nova1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise all three reported isoforms (NP_002506.2
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Evolution of Nova-dependent splicing regulation in the brain.
Jelen N, Ule J, Zivin M, Darnell RB
PLoS genetics 2007 Oct;3(10):1838-47
PLoS genetics 2007 Oct;3(10):1838-47
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nova1 Antibody [NBP1-00221] - Analysis of Nova1 in Human Breast Cancer lysate (35 ug protein in RIPA buffer) using NBP1-00221 at 0.01 ug/mL. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nova1 Antibody [NBP1-00221] - HEK293 overexpressing NOVA1 and probed with (mock transfection in first lane).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nova1 Antibody [NBP1-00221] - Staining of paraffin embedded Human Cerebral Cortex at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nova1 Antibody [NBP1-00221] - Staining of paraffin embedded Human Heart. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nova1 Antibody [NBP1-00221] - Staining of paraffin embedded Human Cortex. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.