PA5-28982

antibody from Invitrogen Antibodies

Targeting: SCAP KIAA0199

Provider product page for PA5-28982

Western blot

Immunohistochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [1]
Comments [0]
Validations
Other assay [3]

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Product number: PA5-28982 - Provider product page
Provider: Invitrogen Antibodies
Product name: SCAP Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: HepG2, MCF-7. Predicted reactivity: Mouse (91%), Rat (95%), Pig (97%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1.43 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

SCAP protein structure - PA5-28982 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 3. Scap expression and cholesterol levels are decreased in hypertrophic chondrocytes . (A) Immunofluorescent staining and in situ hybridization of E16.5 embryos showed that Scap protein was expressed in the round cell zone (resting) and proliferation zone, but was decreased in the hypertrophic zone. Left panel is immunofluorescent staining, middle panels are magnified views of the three zones and the right panels show in situ hybridization. Scale bars: 100 mum. (B) RT-PCR data from microdissected regions of the proliferating zone (PZ) and hypetrophic zone (HZ) of the growth plate showing differential regulation of Scap and other genes involved in intracellular biosynthesis of cholesterol and lipids (from E16.5 limbs, n =8 in each group). Black represents controls; gray represents cells from limbs lacking Scap . (C) Cholesterol levels in the proliferating zone (PZ) and hypetrophic zone (HZ) of the growth plate ( n =4). (D,E) Scap expression micromass cultures also showed that Scap expression was gradually decreased over time as cells progressed through chondrocytic differentiation. D shows RNA data ( n =8) and E a representative western blot. Means and 95% confidence intervals are shown (* P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 4. Loss of Scap in chondrocytes results in a disordered growth plate and severe dwarfism. (A) Immunohistochemistry for SCAP (left) and filipin fluorescent staining (right) for control limbs (top) and for limbs from mice lacking Scap in Col2a1 -expressing cells (bottom), confirming lack of SCAP expression and decreased intracellular cholesterol and lipids in mutant mice. (B,C) Whole-mount and skeletal preparations of representative E12.5 to P0 mice. Top rows show mice expressing Scap in Col2a1 -expressing cells; the bottom rows show mice lacking Scap in these cells ( n >5 for each genotype at each age). P0 images of the spine and limb show a lack of spinal cartilage development and severely shortened long bones. (D) Hematoxylin and Eosin staining of fetal limbs of E16.5 humerus sections with control limbs on the right, limbs from a mouse lacking Scap in one allele in the middle and limbs from a mouse lacking Scap in both alleles in Col2a1 -expressing cells on the left. (E) Magnified views of 16.5 humeri showing views of the resting, proliferating and hypertrophic zones. The mutant limb demonstrates ectopic hypertrophic cells. (F) Alcian Blue staining of the upper limb. (G) Type X collagen staining of the upper limb. (H) BrdU incorporation, with the limbs from mice lacking Scap . (I) Expression of various genes in the mutant and control limbs. Means and 95% confidence intervals are shown (* P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 2. Scap regulates mesenchymal cell proliferation and differentiation. (A) Representative Alcian Blue staining from micromass cultures showing decreased glycosaminoglycan production in limbs from mice with inactivation of Scap in Prx1 -expressing cells ( n =5 mutant and 5 controls). (B) Relative RNA expression comparing micromass cultures lacking Scap in Prx1 -expressing cells with controls, showing decreased expression of markers of chondrogenesis at 10 days ( n =6 mutant and 6 controls). Means and 95% confidence intervals are shown. (C) BrdU uptake in limbs. Graphs of means and 95% confidence intervals are underneath, n =6 for each time point and condition. (D) Scap -deficient mice contained TUNEL-stained cells and cleaved caspase 3 in the central regions of the developing bone, whereas TUNEL-stained cells were restricted to the margins of digital rays ( n =6 for each genotype). Graphs of means and 95% confidence intervals are underneath, n =6 for each, P