PA5-85512

antibody from Invitrogen Antibodies

Targeting: USP3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-85512

Provider

Invitrogen Antibodies

Product name

USP3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Keep as concentrated solution.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of USP3 in A) 293T whole cell lysate, B) A431 whole cell lysate, C) HeLa whole cell lysate using USP3 polyclonal antibody (Product # PA5-85512) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of USP3 in A) 293T whole cell lysate, B) whole cell lysate of human USP3-transfected 293T cells using USP3 polyclonal antibody (Product # PA5-85512) using 30 µg of sample at a dilution of 1:20000. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

USP3 Polyclonal Antibody detects USP3 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg A431 whole cell lysate/extract. C. 30 µg HeLa whole cell lysate/extract.7.5 % SDS-PAGE. USP3 Polyclonal Antibody (Product # PA5-85512) dilution: 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

USP3 Polyclonal Antibody detects USP3 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg whole cell lysate/extract of human USP3-transfected 293T cells.7.5 % SDS-PAGE. USP3 Polyclonal Antibody (Product # PA5-85512) dilution: 1:20,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

USP3 Polyclonal Antibody detects USP3 protein at nucleus by immunofluorescent analysis. Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: USP3 protein stained by USP3 Polyclonal Antibody (Product # PA5-85512) diluted at 1:500. Blue: Hoechst 33342 staining.