12490-1-AP

antibody from Invitrogen Antibodies

Targeting: USP3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12490-1-AP

Provider

Invitrogen Antibodies

Product name

USP3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MECPHLSSS VCIAPDSAKF PNGSPSSWCC SVCRSNKSPW VCLTCSSVHC GRYVNGHAKK HYEDAQVPLT NHKKSEKQDK VQHTVCMDCS SYSTYCYRCD DFVVNDTKLG LVQKVREHLQ NLENSAFTAD RHKKRKLLEN STLNSKLLKV NGSTTAICAT GLRNLGNTCF MNAILQSLSN IEQFCCYFKE LPAVELRNGK TAGRRTYHTR SQGDNNVSLV EEFRKTLCAL WQGSQTAFSP ESLFYVVWKI MPNFRGYQQQ DAHEFMRYLL DHLHLELQGG FNGVSRSAIL QENSTLSASN K (1-300 aa encoded by BC018113)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 12490-1-AP (USP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RAW264.7 cells were subjected to SDS PAGE followed by western blot with 12490-1-AP (USP3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 12490-1-AP (USP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 12490-1-AP (USP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA) fixed HeLa cells using 12490-1-AP (USP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA) fixed HepG2 cells using 12490-1-AP (USP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells using 12490-1-AP (USP3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-USP3 (IP:12490-1-AP, 4ug; Detection:12490-1-AP 1:500) with mouse liver tissue lysate 4000ug.