AF2277
antibody from Novus Biologicals
Targeting: CXCL8
3-10C, AMCF-I, b-ENAP, GCP-1, GCP1, IL-8, IL8, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, SCYB8, TSG-1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF2277 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal CXCL8/IL-8 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects feline IL-8/CXCL8 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant canine IL-8/CXCL8 is observed and 15% cross-reactivity with recombinant porcine IL-8/CXCL8 and recombinant human IL-8/CXCL8 is observed.
- Reactivity
- Feline
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Chemotaxis Induced by IL-8/CXCL8 and Neutral-ization by Feline IL-8/CXCL8 Antibody. Recombinant Feline IL-8/CXCL8 (Catalog # 2277-FL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Feline IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Feline IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2277). The ND50 is typically 0.1-0.4 µg/mL.