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antibody from Novus Biologicals
Targeting: CXCL8
3-10C, AMCF-I, b-ENAP, GCP-1, GCP1, IL-8, IL8, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, SCYB8, TSG-1
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF535 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal CXCL8/IL-8 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IL-8/CXCL8 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human IL-8 is observed.
- Reactivity
- Porcine
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Matrine inhibits IL-1β secretion in primary porcine alveolar macrophages through the MyD88/NF-κB pathway and NLRP3 inflammasome.
Exposure to hog barn dust alters airway epithelial ciliary beating.
Sun P, Sun N, Yin W, Sun Y, Fan K, Guo J, Khan A, He Y, Li H
Veterinary research 2019 Jul 12;50(1):53
Veterinary research 2019 Jul 12;50(1):53
Exposure to hog barn dust alters airway epithelial ciliary beating.
Wyatt TA, Sisson JH, Von Essen SG, Poole JA, Romberger DJ
The European respiratory journal 2008 Jun;31(6):1249-55
The European respiratory journal 2008 Jun;31(6):1249-55
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Chemotaxis Induced by IL-8/CXCL8 and Neutralization by Porcine CXCL8/IL-8 Antibody. Recombinant Porcine IL-8/CXCL8 (Catalog # 535-IN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Porcine IL-8/CXCL8 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF535). The ND50 is typically 1.5-7.5 µg/mL.
