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LearnMAB208-100
antibody from R&D Systems
Targeting: CXCL8
3-10C, AMCF-I, b-ENAP, GCP-1, GCP1, IL-8, IL8, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, SCYB8, TSG-1
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [75]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB208-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-8/CXCL8 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-8/CXCL8 in ELISAs and Western blots. In Western blots, 100% cross-reactivity with recombinant porcine IL-8/CXCL8 and no cross-reactivity with recombinant rat CXCL3/CINC-2 alpha is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P10145- Isotype
- IgG
- Antibody clone number
- 6217
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Differentiated fibrocytes assume a functional mesenchymal phenotype with regenerative potential.
L-selectin expression is regulated by CXCL8-induced reactive oxygen species produced during human neutrophil rolling.
Varicella Zoster Virus Induces Differential Cell-Type Specific Responses in Human Corneal Epithelial Cells and Keratocytes.
Tumoral NOX4 recruits M2 tumor-associated macrophages via ROS/PI3K signaling-dependent various cytokine production to promote NSCLC growth.
Circulating Truncated Alpha-1 Antitrypsin Glycoprotein in Patient Plasma Retains Anti-Inflammatory Capacity.
HSP90 inhibition alters the chemotherapy-driven rearrangement of the oncogenic secretome.
Reactive astrocytic S1P3 signaling modulates the blood-tumor barrier in brain metastases.
Inhibition of IRE1 RNase activity modulates the tumor cell secretome and enhances response to chemotherapy.
DACH1 antagonizes CXCL8 to repress tumorigenesis of lung adenocarcinoma and improve prognosis.
Age-Related Changes to Human Tear Composition.
Reduced microRNA-503 expression augments lung fibroblast VEGF production in chronic obstructive pulmonary disease.
Human Adipose-Derived Mesenchymal Stem Cell-Secreted CXCL1 and CXCL8 Facilitate Breast Tumor Growth By Promoting Angiogenesis.
Neutrophil Membrane Cholesterol Content is a Key Factor in Cystic Fibrosis Lung Disease.
Atorvastatin Promotes Phagocytosis and Attenuates Pro-Inflammatory Response in Human Retinal Pigment Epithelial Cells.
Histone Deacetylase (HDAC) Inhibition Induces IκB Kinase (IKK)-dependent Interleukin-8/CXCL8 Expression in Ovarian Cancer Cells.
Novel augmentation by bufalin of protein kinase C-induced cyclooxygenase-2 and IL-8 production in human breast cancer cells.
Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory "FADDosome" Complex upon TRAIL Stimulation.
Human Pancreatic Cancer Cells Induce a MyD88-Dependent Stromal Response to Promote a Tumor-Tolerant Immune Microenvironment.
Endothelial cell leptin receptors, leptin and interleukin-8 in the pathogenesis of preeclampsia: An in-vitro study.
Upregulated unique long 16 binding protein 1 detected in preeclamptic placenta affects human extravillous trophoblast cell line (HTR-8/SVneo) invasion by modulating the function of uterine natural killer cells.
NOTCH1 mediates a switch between two distinct secretomes during senescence.
Growth Hormone Releasing Peptide-2 Attenuation of Protein Kinase C-Induced Inflammation in Human Ovarian Granulosa Cells.
Tsr Chemoreceptor Interacts With IL-8 Provoking E. coli Transmigration Across Human Lung Epithelial Cells.
Chemokine CCL15 Mediates Migration of Human Bone Marrow-Derived Mesenchymal Stem Cells Toward Hepatocellular Carcinoma.
Loss of RACK1 Promotes Metastasis of Gastric Cancer by Inducing a miR-302c/IL8 Signaling Loop.
Chemotherapy-Derived Inflammatory Responses Accelerate the Formation of Immunosuppressive Myeloid Cells in the Tissue Microenvironment of Human Pancreatic Cancer.
Serum amyloid A chemoattracts immature dendritic cells and indirectly provokes monocyte chemotaxis by induction of cooperating CC and CXC chemokines.
The effect of the decoy molecule PA401 on CXCL8 levels in bronchoalveolar lavage fluid of patients with cystic fibrosis.
Urocortin 2 role in placental and myometrial inflammatory mechanisms at parturition.
CD82/KAI expression prevents IL-8-mediated endothelial gap formation in late-stage melanomas.
Citrullination and proteolytic processing of chemokines by Porphyromonas gingivalis.
Podocytes regulate neutrophil recruitment by glomerular endothelial cells via IL-6-mediated crosstalk.
Interleukin-1-induced activation of the small GTPase Rac1 depends on receptor internalization and regulates gene expression.
Pathological study of archival lung tissues from five fatal cases of avian H5N1 influenza in Vietnam.
Monocytes induce STAT3 activation in human mesenchymal stem cells to promote osteoblast formation.
Daily exposure to dust alters innate immunity.
Macrophages induce differentiation of plasma cells through CXCL10/IP-10.
Neutralization of the IL-17 axis diminishes neutrophil invasion and protects from ischemic stroke.
Interleukin (IL)-4, IL-13, and IL-17A differentially affect the profibrotic and proinflammatory functions of fibrocytes from asthmatic patients.
Semaphorin 4A exerts a proangiogenic effect by enhancing vascular endothelial growth factor-A expression in macrophages.
Protection from RNA and DNA viruses by IL-32.
Skin Inflammation Induced by the Synergistic Action of IL-17A, IL-22, Oncostatin M, IL-1{alpha}, and TNF-{alpha} Recapitulates Some Features of Psoriasis.
Suppression of TNF-α and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27.
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Oxidized {alpha}1-antitrypsin stimulates the release of monocyte chemotactic protein-1 from lung epithelial cells: potential role in emphysema.
Persistent DNA damage signalling triggers senescence-associated inflammatory cytokine secretion.
Expression of CXCL8, CXCR1, and CXCR2 in neurons and glial cells of the human and rabbit retina.
IL-8 as antibody therapeutic target in inflammatory diseases: reduction of clinical activity in palmoplantar pustulosis.
Endogenous IL-32 controls cytokine and HIV-1 production.
Maternal and cord plasma cytokine and chemokine profile in pregnancies complicated by asthma.
Ultrasensitive flow-based immunoassays using single-molecule counting.
L5, the most electronegative subfraction of plasma LDL, induces endothelial vascular cell adhesion molecule 1 and CXC chemokines, which mediate mononuclear leukocyte adhesion.
Tumour necrosis factor-alpha (TNF-alpha) increases nuclear factor kappaB (NFkappaB) activity in and interleukin-8 (IL-8) release from bovine mammary epithelial cells.
Increased ENA-78 in the follicular fluid of patients with endometriosis.
Combination therapy: Synergistic suppression of virus-induced chemokines in airway epithelial cells.
Circulating CD34+ progenitor cells modulate host angiogenesis and inflammation in vivo.
Proteinase-activated receptor2 agonists upregulate granulocyte colony-stimulating factor, IL-8, and VCAM-1 expression in human bronchial fibroblasts.
PAPP-A and osteoprotegerin, together with interleukin-8 and RANTES, are elevated in the peritoneal fluid of women with endometriosis.
Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors.
Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
Interleukin-6 and interleukin-8 in cervical fluid in a population of Swedish women in preterm labor: relationship to microbial invasion of the amniotic fluid, intra-amniotic inflammation, and preterm delivery.
GCP-2/CXCL6 synergizes with other endothelial cell-derived chemokines in neutrophil mobilization and is associated with angiogenesis in gastrointestinal tumors.
The activation of IL-8 receptors in cultured guinea pig Müller glial cells is modified by signals from retinal pigment epithelium.
17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells.
Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis.
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
ELR+ CXC chemokines in human milk.
Interleukin-8 production in tuberculous pleurisy: role of mesothelial cells stimulated by cytokine network involving tumour necrosis factor-alpha and interleukin-1 beta.
Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
Metabolic replacement of kidney function in uremic animals with a bioartificial kidney containing human cells.
Expression of functional CCR and CXCR chemokine receptors in podocytes.
Selective induction of eotaxin release by interleukin-13 or interleukin-4 in human airway smooth muscle cells is synergistic with interleukin-1beta and is mediated by the interleukin-4 receptor alpha-chain.
Il-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation.
Chemoattractant factors in breast milk from allergic and nonallergic mothers.
Ling C, Nishimoto K, Rolfs Z, Smith LM, Frey BL, Welham NV
Science advances 2019 May;5(5):eaav7384
Science advances 2019 May;5(5):eaav7384
L-selectin expression is regulated by CXCL8-induced reactive oxygen species produced during human neutrophil rolling.
Domínguez-Luis MJ, Armas-González E, Herrera-García A, Arce-Franco M, Feria M, Vicente-Manzanares M, Martínez-Ruiz A, Sánchez-Madrid F, Díaz-González F
European journal of immunology 2019 Mar;49(3):386-397
European journal of immunology 2019 Mar;49(3):386-397
Varicella Zoster Virus Induces Differential Cell-Type Specific Responses in Human Corneal Epithelial Cells and Keratocytes.
Como CN, Bubak AN, Blackmon AM, Jones D, Mueller NH, Davidson R, Nagel MA
Investigative ophthalmology & visual science 2019 Feb 1;60(2):704-711
Investigative ophthalmology & visual science 2019 Feb 1;60(2):704-711
Tumoral NOX4 recruits M2 tumor-associated macrophages via ROS/PI3K signaling-dependent various cytokine production to promote NSCLC growth.
Zhang J, Li H, Wu Q, Chen Y, Deng Y, Yang Z, Zhang L, Liu B
Redox biology 2019 Apr;22:101116
Redox biology 2019 Apr;22:101116
Circulating Truncated Alpha-1 Antitrypsin Glycoprotein in Patient Plasma Retains Anti-Inflammatory Capacity.
Reeves EP, Dunlea DM, McQuillan K, O'Dwyer CA, Carroll TP, Saldova R, Akepati PR, Wormald MR, McElvaney OJ, Shutchaidat V, Henry M, Meleady P, Keenan J, Liberti DC, Kotton DN, Rudd PM, Wilson AA, McElvaney NG
Journal of immunology (Baltimore, Md. : 1950) 2019 Apr 15;202(8):2240-2253
Journal of immunology (Baltimore, Md. : 1950) 2019 Apr 15;202(8):2240-2253
HSP90 inhibition alters the chemotherapy-driven rearrangement of the oncogenic secretome.
di Martino S, Amoreo CA, Nuvoli B, Galati R, Strano S, Facciolo F, Alessandrini G, Pass HI, Ciliberto G, Blandino G, De Maria R, Cioce M
Oncogene 2018 Mar;37(10):1369-1385
Oncogene 2018 Mar;37(10):1369-1385
Reactive astrocytic S1P3 signaling modulates the blood-tumor barrier in brain metastases.
Gril B, Paranjape AN, Woditschka S, Hua E, Dolan EL, Hanson J, Wu X, Kloc W, Izycka-Swieszewska E, Duchnowska R, Pęksa R, Biernat W, Jassem J, Nayyar N, Brastianos PK, Hall OM, Peer CJ, Figg WD, Pauly GT, Robinson C, Difilippantonio S, Bialecki E, Metellus P, Schneider JP, Steeg PS
Nature communications 2018 Jul 13;9(1):2705
Nature communications 2018 Jul 13;9(1):2705
Inhibition of IRE1 RNase activity modulates the tumor cell secretome and enhances response to chemotherapy.
Logue SE, McGrath EP, Cleary P, Greene S, Mnich K, Almanza A, Chevet E, Dwyer RM, Oommen A, Legembre P, Godey F, Madden EC, Leuzzi B, Obacz J, Zeng Q, Patterson JB, Jäger R, Gorman AM, Samali A
Nature communications 2018 Aug 15;9(1):3267
Nature communications 2018 Aug 15;9(1):3267
DACH1 antagonizes CXCL8 to repress tumorigenesis of lung adenocarcinoma and improve prognosis.
Liu Q, Li A, Yu S, Qin S, Han N, Pestell RG, Han X, Wu K
Journal of hematology & oncology 2018 Apr 10;11(1):53
Journal of hematology & oncology 2018 Apr 10;11(1):53
Age-Related Changes to Human Tear Composition.
Micera A, Di Zazzo A, Esposito G, Longo R, Foulsham W, Sacco R, Sgrulletta R, Bonini S
Investigative ophthalmology & visual science 2018 Apr 1;59(5):2024-2031
Investigative ophthalmology & visual science 2018 Apr 1;59(5):2024-2031
Reduced microRNA-503 expression augments lung fibroblast VEGF production in chronic obstructive pulmonary disease.
Ikari J, Nelson AJ, Obaid J, Giron-Martinez A, Ikari K, Makino F, Iwasawa S, Gunji Y, Farid M, Wang X, Basma H, Demeo D, Feghali-Bostwick C, Holz O, Rabe K, Liu X, Rennard SI
PloS one 2017;12(9):e0184039
PloS one 2017;12(9):e0184039
Human Adipose-Derived Mesenchymal Stem Cell-Secreted CXCL1 and CXCL8 Facilitate Breast Tumor Growth By Promoting Angiogenesis.
Wang Y, Liu J, Jiang Q, Deng J, Xu F, Chen X, Cheng F, Zhang Y, Yao Y, Xia Z, Xu X, Su X, Huang M, Dai L, Yang Y, Zhang S, Yu D, Zhao RC, Wei Y, Deng H
Stem cells (Dayton, Ohio) 2017 Sep;35(9):2060-2070
Stem cells (Dayton, Ohio) 2017 Sep;35(9):2060-2070
Neutrophil Membrane Cholesterol Content is a Key Factor in Cystic Fibrosis Lung Disease.
White MM, Geraghty P, Hayes E, Cox S, Leitch W, Alfawaz B, Lavelle GM, McElvaney OJ, Flannery R, Keenan J, Meleady P, Henry M, Clynes M, Gunaratnam C, McElvaney NG, Reeves EP
EBioMedicine 2017 Sep;23:173-184
EBioMedicine 2017 Sep;23:173-184
Atorvastatin Promotes Phagocytosis and Attenuates Pro-Inflammatory Response in Human Retinal Pigment Epithelial Cells.
Tian B, Al-Moujahed A, Bouzika P, Hu Y, Notomi S, Tsoka P, Miller JW, Lin H, Vavvas DG
Scientific reports 2017 May 24;7(1):2329
Scientific reports 2017 May 24;7(1):2329
Histone Deacetylase (HDAC) Inhibition Induces IκB Kinase (IKK)-dependent Interleukin-8/CXCL8 Expression in Ovarian Cancer Cells.
Gatla HR, Zou Y, Uddin MM, Singha B, Bu P, Vancura A, Vancurova I
The Journal of biological chemistry 2017 Mar 24;292(12):5043-5054
The Journal of biological chemistry 2017 Mar 24;292(12):5043-5054
Novel augmentation by bufalin of protein kinase C-induced cyclooxygenase-2 and IL-8 production in human breast cancer cells.
Chen HT, Sun D, Peng YC, Kao PH, Wu YL
Innate immunity 2017 Jan;23(1):54-66
Innate immunity 2017 Jan;23(1):54-66
Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory "FADDosome" Complex upon TRAIL Stimulation.
Henry CM, Martin SJ
Molecular cell 2017 Feb 16;65(4):715-729.e5
Molecular cell 2017 Feb 16;65(4):715-729.e5
Human Pancreatic Cancer Cells Induce a MyD88-Dependent Stromal Response to Promote a Tumor-Tolerant Immune Microenvironment.
Delitto D, Delitto AE, DiVita BB, Pham K, Han S, Hartlage ER, Newby BN, Gerber MH, Behrns KE, Moldawer LL, Thomas RM, George TJ Jr, Brusko TM, Mathews CE, Liu C, Trevino JG, Hughes SJ, Wallet SM
Cancer research 2017 Feb 1;77(3):672-683
Cancer research 2017 Feb 1;77(3):672-683
Endothelial cell leptin receptors, leptin and interleukin-8 in the pathogenesis of preeclampsia: An in-vitro study.
Arlıer S
Turkish journal of obstetrics and gynecology 2017 Dec;14(4):220-227
Turkish journal of obstetrics and gynecology 2017 Dec;14(4):220-227
Upregulated unique long 16 binding protein 1 detected in preeclamptic placenta affects human extravillous trophoblast cell line (HTR-8/SVneo) invasion by modulating the function of uterine natural killer cells.
Liu J, Song G, Lin X, Pang X, Meng T
Experimental and therapeutic medicine 2017 Apr;13(4):1447-1455
Experimental and therapeutic medicine 2017 Apr;13(4):1447-1455
NOTCH1 mediates a switch between two distinct secretomes during senescence.
Hoare M, Ito Y, Kang TW, Weekes MP, Matheson NJ, Patten DA, Shetty S, Parry AJ, Menon S, Salama R, Antrobus R, Tomimatsu K, Howat W, Lehner PJ, Zender L, Narita M
Nature cell biology 2016 Sep;18(9):979-92
Nature cell biology 2016 Sep;18(9):979-92
Growth Hormone Releasing Peptide-2 Attenuation of Protein Kinase C-Induced Inflammation in Human Ovarian Granulosa Cells.
Chao YN, Sun D, Peng YC, Wu YL
International journal of molecular sciences 2016 Aug 19;17(8)
International journal of molecular sciences 2016 Aug 19;17(8)
Tsr Chemoreceptor Interacts With IL-8 Provoking E. coli Transmigration Across Human Lung Epithelial Cells.
Han B, Li M, Xu Y, Islam D, Khang J, Del Sorbo L, Lee W, Szaszi K, Zhong N, Slutsky AS, Li Y, Zhang H
Scientific reports 2016 Aug 10;6:31087
Scientific reports 2016 Aug 10;6:31087
Chemokine CCL15 Mediates Migration of Human Bone Marrow-Derived Mesenchymal Stem Cells Toward Hepatocellular Carcinoma.
Gao Y, Zhou Z, Lu S, Huang X, Zhang C, Jiang R, Yao A, Sun B, Wang X
Stem cells (Dayton, Ohio) 2016 Apr;34(4):1112-22
Stem cells (Dayton, Ohio) 2016 Apr;34(4):1112-22
Loss of RACK1 Promotes Metastasis of Gastric Cancer by Inducing a miR-302c/IL8 Signaling Loop.
Chen L, Min L, Wang X, Zhao J, Chen H, Qin J, Chen W, Shen Z, Tang Z, Gan Q, Ruan Y, Sun Y, Qin X, Gu J
Cancer research 2015 Sep 15;75(18):3832-41
Cancer research 2015 Sep 15;75(18):3832-41
Chemotherapy-Derived Inflammatory Responses Accelerate the Formation of Immunosuppressive Myeloid Cells in the Tissue Microenvironment of Human Pancreatic Cancer.
Takeuchi S, Baghdadi M, Tsuchikawa T, Wada H, Nakamura T, Abe H, Nakanishi S, Usui Y, Higuchi K, Takahashi M, Inoko K, Sato S, Takano H, Shichinohe T, Seino K, Hirano S
Cancer research 2015 Jul 1;75(13):2629-40
Cancer research 2015 Jul 1;75(13):2629-40
Serum amyloid A chemoattracts immature dendritic cells and indirectly provokes monocyte chemotaxis by induction of cooperating CC and CXC chemokines.
Gouwy M, De Buck M, Pörtner N, Opdenakker G, Proost P, Struyf S, Van Damme J
European journal of immunology 2015 Jan;45(1):101-12
European journal of immunology 2015 Jan;45(1):101-12
The effect of the decoy molecule PA401 on CXCL8 levels in bronchoalveolar lavage fluid of patients with cystic fibrosis.
McElvaney OJ, O'Reilly N, White M, Lacey N, Pohl K, Gerlza T, Bergin DA, Kerr H, McCarthy C, O'Brien ME, Adage T, Kungl AJ, Reeves EP, McElvaney NG
Molecular immunology 2015 Feb;63(2):550-8
Molecular immunology 2015 Feb;63(2):550-8
Urocortin 2 role in placental and myometrial inflammatory mechanisms at parturition.
Voltolini C, Battersby S, Novembri R, Torricelli M, Severi FM, Petraglia F, Norman JE
Endocrinology 2015 Feb;156(2):670-9
Endocrinology 2015 Feb;156(2):670-9
CD82/KAI expression prevents IL-8-mediated endothelial gap formation in late-stage melanomas.
Khanna P, Chung CY, Neves RI, Robertson GP, Dong C
Oncogene 2014 May 29;33(22):2898-908
Oncogene 2014 May 29;33(22):2898-908
Citrullination and proteolytic processing of chemokines by Porphyromonas gingivalis.
Moelants EA, Loozen G, Mortier A, Martens E, Opdenakker G, Mizgalska D, Szmigielski B, Potempa J, Van Damme J, Teughels W, Proost P
Infection and immunity 2014 Jun;82(6):2511-9
Infection and immunity 2014 Jun;82(6):2511-9
Podocytes regulate neutrophil recruitment by glomerular endothelial cells via IL-6-mediated crosstalk.
Kuravi SJ, McGettrick HM, Satchell SC, Saleem MA, Harper L, Williams JM, Rainger GE, Savage CO
Journal of immunology (Baltimore, Md. : 1950) 2014 Jul 1;193(1):234-43
Journal of immunology (Baltimore, Md. : 1950) 2014 Jul 1;193(1):234-43
Interleukin-1-induced activation of the small GTPase Rac1 depends on receptor internalization and regulates gene expression.
Windheim M, Hansen B
Cellular signalling 2014 Jan;26(1):49-55
Cellular signalling 2014 Jan;26(1):49-55
Pathological study of archival lung tissues from five fatal cases of avian H5N1 influenza in Vietnam.
Nakajima N, Van Tin N, Sato Y, Thach HN, Katano H, Diep PH, Kumasaka T, Thuy NT, Hasegawa H, San LT, Kawachi S, Liem NT, Suzuki K, Sata T
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2013 Mar;26(3):357-69
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2013 Mar;26(3):357-69
Monocytes induce STAT3 activation in human mesenchymal stem cells to promote osteoblast formation.
Nicolaidou V, Wong MM, Redpath AN, Ersek A, Baban DF, Williams LM, Cope AP, Horwood NJ
PloS one 2012;7(7):e39871
PloS one 2012;7(7):e39871
Daily exposure to dust alters innate immunity.
Sahlander K, Larsson K, Palmberg L
PloS one 2012;7(2):e31646
PloS one 2012;7(2):e31646
Macrophages induce differentiation of plasma cells through CXCL10/IP-10.
Xu W, Joo H, Clayton S, Dullaers M, Herve MC, Blankenship D, De La Morena MT, Balderas R, Picard C, Casanova JL, Pascual V, Oh S, Banchereau J
The Journal of experimental medicine 2012 Sep 24;209(10):1813-23, S1-2
The Journal of experimental medicine 2012 Sep 24;209(10):1813-23, S1-2
Neutralization of the IL-17 axis diminishes neutrophil invasion and protects from ischemic stroke.
Gelderblom M, Weymar A, Bernreuther C, Velden J, Arunachalam P, Steinbach K, Orthey E, Arumugam TV, Leypoldt F, Simova O, Thom V, Friese MA, Prinz I, Hölscher C, Glatzel M, Korn T, Gerloff C, Tolosa E, Magnus T
Blood 2012 Nov 1;120(18):3793-802
Blood 2012 Nov 1;120(18):3793-802
Interleukin (IL)-4, IL-13, and IL-17A differentially affect the profibrotic and proinflammatory functions of fibrocytes from asthmatic patients.
Bellini A, Marini MA, Bianchetti L, Barczyk M, Schmidt M, Mattoli S
Mucosal immunology 2012 Mar;5(2):140-9
Mucosal immunology 2012 Mar;5(2):140-9
Semaphorin 4A exerts a proangiogenic effect by enhancing vascular endothelial growth factor-A expression in macrophages.
Meda C, Molla F, De Pizzol M, Regano D, Maione F, Capano S, Locati M, Mantovani A, Latini R, Bussolino F, Giraudo E
Journal of immunology (Baltimore, Md. : 1950) 2012 Apr 15;188(8):4081-92
Journal of immunology (Baltimore, Md. : 1950) 2012 Apr 15;188(8):4081-92
Protection from RNA and DNA viruses by IL-32.
Zepp JA, Nold-Petry CA, Dinarello CA, Nold MF
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 1;186(7):4110-8
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 1;186(7):4110-8
Skin Inflammation Induced by the Synergistic Action of IL-17A, IL-22, Oncostatin M, IL-1{alpha}, and TNF-{alpha} Recapitulates Some Features of Psoriasis.
Guilloteau K, Paris I, Pedretti N, Boniface K, Juchaux F, Huguier V, Guillet G, Bernard FX, Lecron JC, Morel F
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):5263-5270
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):5263-5270
Suppression of TNF-α and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27.
Kalliolias GD, Gordon RA, Ivashkiv LB
Journal of immunology (Baltimore, Md. : 1950) 2010 Dec 1;185(11):7047-56
Journal of immunology (Baltimore, Md. : 1950) 2010 Dec 1;185(11):7047-56
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Hu H, Kwun J, Aizenstein BD, Knechtle SJ
Transplantation 2009 Jun 27;87(12):1814-20
Transplantation 2009 Jun 27;87(12):1814-20
Oxidized {alpha}1-antitrypsin stimulates the release of monocyte chemotactic protein-1 from lung epithelial cells: potential role in emphysema.
Li Z, Alam S, Wang J, Sandstrom CS, Janciauskiene S, Mahadeva R
American journal of physiology. Lung cellular and molecular physiology 2009 Aug;297(2):L388-400
American journal of physiology. Lung cellular and molecular physiology 2009 Aug;297(2):L388-400
Persistent DNA damage signalling triggers senescence-associated inflammatory cytokine secretion.
Rodier F, Coppé JP, Patil CK, Hoeijmakers WA, Muñoz DP, Raza SR, Freund A, Campeau E, Davalos AR, Campisi J
Nature cell biology 2009 Aug;11(8):973-9
Nature cell biology 2009 Aug;11(8):973-9
Expression of CXCL8, CXCR1, and CXCR2 in neurons and glial cells of the human and rabbit retina.
Goczalik I, Ulbricht E, Hollborn M, Raap M, Uhlmann S, Weick M, Pannicke T, Wiedemann P, Bringmann A, Reichenbach A, Francke M
Investigative ophthalmology & visual science 2008 Oct;49(10):4578-89
Investigative ophthalmology & visual science 2008 Oct;49(10):4578-89
IL-8 as antibody therapeutic target in inflammatory diseases: reduction of clinical activity in palmoplantar pustulosis.
Skov L, Beurskens FJ, Zachariae CO, Reitamo S, Teeling J, Satijn D, Knudsen KM, Boot EP, Hudson D, Baadsgaard O, Parren PW, van de Winkel JG
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 1;181(1):669-79
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 1;181(1):669-79
Endogenous IL-32 controls cytokine and HIV-1 production.
Nold MF, Nold-Petry CA, Pott GB, Zepp JA, Saavedra MT, Kim SH, Dinarello CA
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 1;181(1):557-65
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 1;181(1):557-65
Maternal and cord plasma cytokine and chemokine profile in pregnancies complicated by asthma.
Osei-Kumah A, Smith R, Clifton VL
Cytokine 2008 Aug;43(2):187-93
Cytokine 2008 Aug;43(2):187-93
Ultrasensitive flow-based immunoassays using single-molecule counting.
Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
Clinical chemistry 2007 Nov;53(11):1990-5
Clinical chemistry 2007 Nov;53(11):1990-5
L5, the most electronegative subfraction of plasma LDL, induces endothelial vascular cell adhesion molecule 1 and CXC chemokines, which mediate mononuclear leukocyte adhesion.
Abe Y, Fornage M, Yang CY, Bui-Thanh NA, Wise V, Chen HH, Rangaraj G, Ballantyne CM
Atherosclerosis 2007 May;192(1):56-66
Atherosclerosis 2007 May;192(1):56-66
Tumour necrosis factor-alpha (TNF-alpha) increases nuclear factor kappaB (NFkappaB) activity in and interleukin-8 (IL-8) release from bovine mammary epithelial cells.
Fitzgerald DC, Meade KG, McEvoy AN, Lillis L, Murphy EP, MacHugh DE, Baird AW
Veterinary immunology and immunopathology 2007 Mar 15;116(1-2):59-68
Veterinary immunology and immunopathology 2007 Mar 15;116(1-2):59-68
Increased ENA-78 in the follicular fluid of patients with endometriosis.
Wunder DM, Mueller MD, Birkhäuser MH, Bersinger NA
Acta obstetricia et gynecologica Scandinavica 2006;85(3):336-42
Acta obstetricia et gynecologica Scandinavica 2006;85(3):336-42
Combination therapy: Synergistic suppression of virus-induced chemokines in airway epithelial cells.
Edwards MR, Johnson MW, Johnston SL
American journal of respiratory cell and molecular biology 2006 May;34(5):616-24
American journal of respiratory cell and molecular biology 2006 May;34(5):616-24
Circulating CD34+ progenitor cells modulate host angiogenesis and inflammation in vivo.
Popa ER, Harmsen MC, Tio RA, van der Strate BW, Brouwer LA, Schipper M, Koerts J, De Jongste MJ, Hazenberg A, Hendriks M, van Luyn MJ
Journal of molecular and cellular cardiology 2006 Jul;41(1):86-96
Journal of molecular and cellular cardiology 2006 Jul;41(1):86-96
Proteinase-activated receptor2 agonists upregulate granulocyte colony-stimulating factor, IL-8, and VCAM-1 expression in human bronchial fibroblasts.
Ramachandran R, Morice AH, Compton SJ
American journal of respiratory cell and molecular biology 2006 Jul;35(1):133-41
American journal of respiratory cell and molecular biology 2006 Jul;35(1):133-41
PAPP-A and osteoprotegerin, together with interleukin-8 and RANTES, are elevated in the peritoneal fluid of women with endometriosis.
Bersinger NA, von Roten S, Wunder DM, Raio L, Dreher E, Mueller MD
American journal of obstetrics and gynecology 2006 Jul;195(1):103-8
American journal of obstetrics and gynecology 2006 Jul;195(1):103-8
Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors.
Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjöstedt A, Edebro H, Forsman M, Byström M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK
Infection and immunity 2006 Dec;74(12):6730-8
Infection and immunity 2006 Dec;74(12):6730-8
Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
Clinical immunology (Orlando, Fla.) 2006 Aug;120(2):220-31
Clinical immunology (Orlando, Fla.) 2006 Aug;120(2):220-31
IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
Journal of immunology (Baltimore, Md. : 1950) 2005 Nov 1;175(9):6177-89
Journal of immunology (Baltimore, Md. : 1950) 2005 Nov 1;175(9):6177-89
Interleukin-6 and interleukin-8 in cervical fluid in a population of Swedish women in preterm labor: relationship to microbial invasion of the amniotic fluid, intra-amniotic inflammation, and preterm delivery.
Holst RM, Mattsby-Baltzer I, Wennerholm UB, Hagberg H, Jacobsson B
Acta obstetricia et gynecologica Scandinavica 2005 Jun;84(6):551-7
Acta obstetricia et gynecologica Scandinavica 2005 Jun;84(6):551-7
GCP-2/CXCL6 synergizes with other endothelial cell-derived chemokines in neutrophil mobilization and is associated with angiogenesis in gastrointestinal tumors.
Gijsbers K, Gouwy M, Struyf S, Wuyts A, Proost P, Opdenakker G, Penninckx F, Ectors N, Geboes K, Van Damme J
Experimental cell research 2005 Feb 15;303(2):331-42
Experimental cell research 2005 Feb 15;303(2):331-42
The activation of IL-8 receptors in cultured guinea pig Müller glial cells is modified by signals from retinal pigment epithelium.
Malgorzata Goczalik I, Raap M, Weick M, Milenkovic I, Heidmann J, Enzmann V, Wiedemann P, Reichenbach A, Francke M
Journal of neuroimmunology 2005 Apr;161(1-2):49-60
Journal of neuroimmunology 2005 Apr;161(1-2):49-60
17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells.
Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA
Blood 2004 Sep 1;104(5):1404-10
Blood 2004 Sep 1;104(5):1404-10
Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis.
Phillips TM
Electrophoresis 2004 Jun;25(10-11):1652-9
Electrophoresis 2004 Jun;25(10-11):1652-9
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
Copeland S, Siddiqui J, Remick D
Journal of immunological methods 2004 Jan;284(1-2):99-106
Journal of immunological methods 2004 Jan;284(1-2):99-106
ELR+ CXC chemokines in human milk.
Maheshwari A, Christensen RD, Calhoun DA
Cytokine 2003 Nov 7;24(3):91-102
Cytokine 2003 Nov 7;24(3):91-102
Interleukin-8 production in tuberculous pleurisy: role of mesothelial cells stimulated by cytokine network involving tumour necrosis factor-alpha and interleukin-1 beta.
Park JS, Kim YS, Jee YK, Myong NH, Lee KY
Scandinavian journal of immunology 2003 May;57(5):463-9
Scandinavian journal of immunology 2003 May;57(5):463-9
Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
Journal of immunology (Baltimore, Md. : 1950) 2002 Nov 1;169(9):5244-51
Journal of immunology (Baltimore, Md. : 1950) 2002 Nov 1;169(9):5244-51
Metabolic replacement of kidney function in uremic animals with a bioartificial kidney containing human cells.
Humes HD, Fissell WH, Weitzel WF, Buffington DA, Westover AJ, MacKay SM, Gutierrez JM
American journal of kidney diseases : the official journal of the National Kidney Foundation 2002 May;39(5):1078-87
American journal of kidney diseases : the official journal of the National Kidney Foundation 2002 May;39(5):1078-87
Expression of functional CCR and CXCR chemokine receptors in podocytes.
Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstädt H
Journal of immunology (Baltimore, Md. : 1950) 2002 Jun 15;168(12):6244-52
Journal of immunology (Baltimore, Md. : 1950) 2002 Jun 15;168(12):6244-52
Selective induction of eotaxin release by interleukin-13 or interleukin-4 in human airway smooth muscle cells is synergistic with interleukin-1beta and is mediated by the interleukin-4 receptor alpha-chain.
Hirst SJ, Hallsworth MP, Peng Q, Lee TH
American journal of respiratory and critical care medicine 2002 Apr 15;165(8):1161-71
American journal of respiratory and critical care medicine 2002 Apr 15;165(8):1161-71
Il-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation.
Hurst SM, Wilkinson TS, McLoughlin RM, Jones S, Horiuchi S, Yamamoto N, Rose-John S, Fuller GM, Topley N, Jones SA
Immunity 2001 Jun;14(6):705-14
Immunity 2001 Jun;14(6):705-14
Chemoattractant factors in breast milk from allergic and nonallergic mothers.
Böttcher MF, Jenmalm MC, Björkstén B, Garofalo RP
Pediatric research 2000 May;47(5):592-7
Pediatric research 2000 May;47(5):592-7
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-8/CXCL8 in Human PBMCs. IL-8/CXCL8 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by IL-8/CXCL8 and Neutralization by Human IL-8/CXCL8 Antibody. Recombinant Human IL-8/CXCL8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208). The ND50 is typically 0.08-0.4 µg/mL.
