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LearnMAB535
antibody from R&D Systems
Targeting: CXCL8
3-10C, AMCF-I, b-ENAP, GCP-1, GCP1, IL-8, IL8, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, SCYB8, TSG-1
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB535 - Provider product page
- Provider
- R&D Systems
- Product name
- Porcine IL-8/CXCL8 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects porcine IL-8/CXCL8 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) CXCL8 or rhCXCL1, 2, 3, 5, 6, 7, 9, 10, 11, 12, 13, recombinant mouse CXCL1, 2, 6, 9, 10, 12, 13, recombinant rat CXCL1, 2 or 3 is observed.
- Reactivity
- Porcine
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
CAA43461- Isotype
- IgG
- Antibody clone number
- 105115
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Characterization of a Broadly Reactive Anti-CD40 Agonistic Monoclonal Antibody for Potential Use as an Adjuvant.
Classical swine fever virus induces proinflammatory cytokines and tissue factor expression and inhibits apoptosis and interferon synthesis during the establishment of long-term infection of porcine vascular endothelial cells.
Martin C, Waghela SD, Lokhandwala S, Ambrus A, Bray J, Vuong C, Vinodkumar V, Dominowski PJ, Rai S, Mwangi D, Foss DL, Mwangi W
PloS one 2017;12(1):e0170504
PloS one 2017;12(1):e0170504
Classical swine fever virus induces proinflammatory cytokines and tissue factor expression and inhibits apoptosis and interferon synthesis during the establishment of long-term infection of porcine vascular endothelial cells.
Bensaude E, Turner JL, Wakeley PR, Sweetman DA, Pardieu C, Drew TW, Wileman T, Powell PP
The Journal of general virology 2004 Apr;85(Pt 4):1029-37
The Journal of general virology 2004 Apr;85(Pt 4):1029-37
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by IL-8/CXCL8 and Neutralization by Porcine IL-8/CXCL8 Antibody. Recombinant Porcine IL-8/CXCL8 (Catalog # 535-IN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Porcine IL-8/CXCL8 (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine CXCL8/IL-8 Monoclonal Antibody (Catalog # MAB535). The ND50 is typically 0.2-1.0 µg/mL.
