PB9621

antibody from Boster Biological Technology

Targeting: CXCL8 3-10C, AMCF-I, b-ENAP, GCP-1, GCP1, IL-8, IL8, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, SCYB8, TSG-1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

PB9621

Provider

Boster Biological Technology

Product name

Anti-IL8/CXCL8 Antibody Picoband™

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for Interleukin-8(CXCL8) detection. Tested with WB, IHC-P, ELISA in Human.

Reactivity

Human

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Suramin ameliorates osteoarthritis by acting on the Nrf2/HO-1 and NF-κB signaling pathways in chondrocytes and promoting M2 polarization in macrophages.

Shen PC, Huang SH, Liu ZM, Lu CC, Chou SH, Tien YC
International immunopharmacology 2023 Jul;120:110295

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The role of glycogen synthase kinase 3 beta in brain injury induced by myocardial ischemia/reperfusion injury in a rat model of diabetes mellitus.

Zhao B, Gao WW, Liu YJ, Jiang M, Liu L, Yuan Q, Hou JB, Xia ZY
Neural regeneration research 2017 Oct;12(10):1632-1639

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Comprehensive evaluation of decellularized porcine corneal after clinical transplantation.

Shi Y, Bikkuzin T, Song Z, Jin X, Jin H, Li X, Zhang H
Xenotransplantation 2017 Nov;24(6)

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Myocardial ischemic post-conditioning protects the lung against myocardial ischemia/reperfusion-induced damage by activating GSK-3β.

Gao W, Zhao B, Liu L, Yuan Q, Wu X, Xia Z
Acta cirurgica brasileira 2017 May;32(5):376-387

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Upregulation of Toll-like receptor 2 expression in colorectal cancer infected by human cytomegalovirus.

Li X, Qian D, Ju F, Wang B
Oncology letters 2015 Jan;9(1):365-370

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Double-stranded RNA transcribed from vector-based oligodeoxynucleotide acts as transcription factor decoy.

Xiao X, Gang Y, Wang H, Wang J, Zhao L, Xu L, Liu Z
Biochemical and biophysical research communications 2015 Feb 6;457(2):221-6

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Distribution change of mast cells in human nasal polyps.

Zhang G, Shao J, Su C, Zhao X, Wang X, Sun X, Shi W, Sun P, Yao Z, Yang J
Anatomical record (Hoboken, N.J. : 2007) 2012 May;295(5):758-63

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of IL-8 using anti-IL-8 antibody (PB9621). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-8 antigen affinity purified polyclonal antibody (Catalog # PB9621) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-8 at approximately 34KD. The expected band size for IL-8 is at 11KD.

Additional image