48-8088-42
antibody from Invitrogen Antibodies
Targeting: CXCL8
3-10C, AMCF-I, b-ENAP, GCP-1, GCP1, IL-8, IL8, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, SCYB8, TSG-1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 48-8088-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-8 (1-77) (CXCL8) Monoclonal Antibody (8CH), eFluor™ 450, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Description: This 8CH monoclonal antibody reacts with human IL-8 (CXCL8), a pro-inflammatory CXC chemokine. It is synthesized as a 99 amino acid precursor protein that is further processed into one of four isoforms, with the most common being 72 or 77 amino acids in length. IL-8(77) is secreted primarily by endothelial cells and is thought to be a less potent neutrophil activator than the other forms. It is present at high levels during fetal development, where it mediates angiogenesis rather than inflammation. The predominant form present in adults is IL-8(72), which is expressed by monocytes, macrophages, epithelial cells, and fibroblasts in response to inflammatory stimuli, environmental stress, and steroid hormones. IL-8(72) is essential for the activation and recruitment of neutrophils to sites of inflammation, and has also been found to influence T cell migration. Signaling occurs through the G-protein coupled receptors CXCR1 or CXCR2. IL-8 transcripts are often upregulated in tumors, and it is associated with tumor angiogenesis and metastasis. Applications Reported: This 8CH antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 8CH antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 8CH
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Normal human peripheral blood cells unstimulated (left) or stimulated with LPS in the presence of Protein Transport Inhibitor Cocktail (Product # 00-4980-03), stained with Anti-Human CD13 PE-Cy7 (Product # 25-0138-42) followed by fixation and intracellular staining with and Anti-Human IL-8 eFluor® 450. Cells in the monocyte gate were used for analysis.