BMS136
antibody from Invitrogen Antibodies
Targeting: CXCL8
3-10C, AMCF-I, b-ENAP, GCP-1, GCP1, IL-8, IL8, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, SCYB8, TSG-1
Antibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Other assay [1]
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- Product number
- BMS136 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-8 (1-77) (CXCL8) Monoclonal Antibody (NAP II), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The antibody can be used to stain acetone-fixed cryostat sections or cell smears. BMS136FI is suitable for intracellular staining of IL-8. Interleukin-8 selectively stimulates the ability of neutrophils and T-lymphocytes to invade injured or inflamed tissue. IL-8 also known as CXCL8 that is purified from peripheral blood monocytes is a 10 kDa protein showing at least 4 variant forms (77, 72, 70, and 69 amino acids, respectively) which differ in the length of the N-terminal and readily form dimers in solution. Two cystin-bridges are essential for the biological activity of IL-8. Exogenous stimuli like LPS, but also IL-1, TNF-alpha and TNF-beta induce the secretion of IL-8 in a variety of different cell types including monocytes, endothelial and epithelial cells, peripheral blood mononuclear cells, dermal fibroblasts, keratinocytes, neutrophils, hepatocytes, synovial cells, and T-lymphocytes. The activities of IL-8 are not species-specific. Human IL-8 is also active in rodent and rabbit cells and differs from all other cytokines in its ability to specifically activate neutrophil granulocytes. This chemokine activates multiple intracellular signaling pathways downstream of two cell-surface, G protein-coupled receptors (CXCR1 and CXCR2). The property of IL-8 to stimulate movement of neutrophils across endothelial monolayers in vitro supports the concept of a central role for this molecule in the accumulation of neutrophils at inflammatory lesions in vivo. Applications Tested: Immunohistochemistry (Frozen), Flow Cytometry (Intracellular Staining). Purity: >99%.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- NAP II
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C
Submitted references The splicing factor U2AF1 contributes to cancer progression through a noncanonical role in translation regulation.
CXCR2 modulates bone marrow vascular repair and haematopoietic recovery post-transplant.
Palangat M, Anastasakis DG, Fei DL, Lindblad KE, Bradley R, Hourigan CS, Hafner M, Larson DR
Genes & development 2019 May 1;33(9-10):482-497
Genes & development 2019 May 1;33(9-10):482-497
CXCR2 modulates bone marrow vascular repair and haematopoietic recovery post-transplant.
Hale SJ, Hale AB, Zhang Y, Sweeney D, Fisher N, van der Garde M, Grabowska R, Pepperell E, Channon K, Martin-Rendon E, Watt SM
British journal of haematology 2015 May;169(4):552-64
British journal of haematology 2015 May;169(4):552-64
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- Invitrogen Antibodies (provider)
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