AF-208-NA
antibody from R&D Systems
Targeting: CXCL8
3-10C, AMCF-I, b-ENAP, GCP-1, GCP1, IL-8, IL8, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, SCYB8, TSG-1
Antibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF-208-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-8/CXCL8 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IL-8/CXCL8 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant canine IL-8/CXCL8, recombinant feline IL-8/CXCL8 and recombinant porcine IL-8/CXCL8 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P10145
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Senescence-associated IL-6 and IL-8 cytokines induce a self- and cross-reinforced senescence/inflammatory milieu strengthening tumorigenic capabilities in the MCF-7 breast cancer cell line.
Cigarette smoking increases bronchial mucosal IL-17A expression in asthmatics, which acts in concert with environmental aeroallergens to engender neutrophilic inflammation.
Necrotic cells influence migration and invasion of glioblastoma via NF-κB/AP-1-mediated IL-8 regulation.
Functional characterization of IL-17F as a selective neutrophil attractant in psoriasis.
Estradiol increases IL-8 secretion of normal human breast tissue and breast cancer in vivo.
Estradiol increases IL-8 secretion of normal human breast tissue and breast cancer in vivo.
The presence of a matrix-derived neutrophil chemoattractant in bronchiolitis obliterans syndrome after lung transplantation.
Influence of eicosapentaenoic acid, an omega-3 fatty acid, on ultraviolet-B generation of prostaglandin-E2 and proinflammatory cytokines interleukin-1 beta, tumor necrosis factor-alpha, interleukin-6 and interleukin-8 in human skin in vivo.
Arenavirus-mediated liver pathology: acute lymphocytic choriomeningitis virus infection of rhesus macaques is characterized by high-level interleukin-6 expression and hepatocyte proliferation.
Arenavirus-mediated liver pathology: acute lymphocytic choriomeningitis virus infection of rhesus macaques is characterized by high-level interleukin-6 expression and hepatocyte proliferation.
Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
Ortiz-Montero P, Londoño-Vallejo A, Vernot JP
Cell communication and signaling : CCS 2017 May 4;15(1):17
Cell communication and signaling : CCS 2017 May 4;15(1):17
Cigarette smoking increases bronchial mucosal IL-17A expression in asthmatics, which acts in concert with environmental aeroallergens to engender neutrophilic inflammation.
Siew LQC, Wu SY, Ying S, Corrigan CJ
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 2017 Jun;47(6):740-750
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 2017 Jun;47(6):740-750
Necrotic cells influence migration and invasion of glioblastoma via NF-κB/AP-1-mediated IL-8 regulation.
Ahn SH, Park H, Ahn YH, Kim S, Cho MS, Kang JL, Choi YH
Scientific reports 2016 Apr 14;6:24552
Scientific reports 2016 Apr 14;6:24552
Functional characterization of IL-17F as a selective neutrophil attractant in psoriasis.
Watanabe H, Kawaguchi M, Fujishima S, Ogura M, Matsukura S, Takeuchi H, Ohba M, Sueki H, Kokubu F, Hizawa N, Adachi M, Huang SK, Iijima M
The Journal of investigative dermatology 2009 Mar;129(3):650-6
The Journal of investigative dermatology 2009 Mar;129(3):650-6
Estradiol increases IL-8 secretion of normal human breast tissue and breast cancer in vivo.
Bendrik C, Dabrosin C
Journal of immunology (Baltimore, Md. : 1950) 2009 Jan 1;182(1):371-8
Journal of immunology (Baltimore, Md. : 1950) 2009 Jan 1;182(1):371-8
Estradiol increases IL-8 secretion of normal human breast tissue and breast cancer in vivo.
Bendrik C, Dabrosin C
Journal of immunology (Baltimore, Md. : 1950) 2009 Jan 1;182(1):371-8
Journal of immunology (Baltimore, Md. : 1950) 2009 Jan 1;182(1):371-8
The presence of a matrix-derived neutrophil chemoattractant in bronchiolitis obliterans syndrome after lung transplantation.
Hardison MT, Galin FS, Calderon CE, Djekic UV, Parker SB, Wille KM, Jackson PL, Oster RA, Young KR, Blalock JE, Gaggar A
Journal of immunology (Baltimore, Md. : 1950) 2009 Apr 1;182(7):4423-31
Journal of immunology (Baltimore, Md. : 1950) 2009 Apr 1;182(7):4423-31
Influence of eicosapentaenoic acid, an omega-3 fatty acid, on ultraviolet-B generation of prostaglandin-E2 and proinflammatory cytokines interleukin-1 beta, tumor necrosis factor-alpha, interleukin-6 and interleukin-8 in human skin in vivo.
Shahbakhti H, Watson RE, Azurdia RM, Ferreira CZ, Garmyn M, Rhodes LE
Photochemistry and photobiology 2004 Sep-Oct;80(2):231-5
Photochemistry and photobiology 2004 Sep-Oct;80(2):231-5
Arenavirus-mediated liver pathology: acute lymphocytic choriomeningitis virus infection of rhesus macaques is characterized by high-level interleukin-6 expression and hepatocyte proliferation.
Lukashevich IS, Tikhonov I, Rodas JD, Zapata JC, Yang Y, Djavani M, Salvato MS
Journal of virology 2003 Feb;77(3):1727-37
Journal of virology 2003 Feb;77(3):1727-37
Arenavirus-mediated liver pathology: acute lymphocytic choriomeningitis virus infection of rhesus macaques is characterized by high-level interleukin-6 expression and hepatocyte proliferation.
Lukashevich IS, Tikhonov I, Rodas JD, Zapata JC, Yang Y, Djavani M, Salvato MS
Journal of virology 2003 Feb;77(3):1727-37
Journal of virology 2003 Feb;77(3):1727-37
Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM
Journal of immunology (Baltimore, Md. : 1950) 2002 Dec 1;169(11):6515-21
Journal of immunology (Baltimore, Md. : 1950) 2002 Dec 1;169(11):6515-21
Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM
Journal of immunology (Baltimore, Md. : 1950) 2002 Dec 1;169(11):6515-21
Journal of immunology (Baltimore, Md. : 1950) 2002 Dec 1;169(11):6515-21
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-8/CXCL8 in Human PBMCs. IL-8/CXCL8 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin using 10 µg/mL Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-8/CXCL8 in Human Tonsil. IL-8/CXCL8 was detected in immersion fixed paraffin-embedded sections of human tonsil (surgically removed due to severe EBV-induced mononucleosis) using Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA) overnight at 4 °C. Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by IL-8/CXCL8 and Neutralization by Human IL-8/CXCL8 Antibody. Recombinant Human IL-8/CXCL8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA). The ND50 is typically 0.1-0.5 µg/mL.