Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [1]
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Validation data
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- Product number
- PA5-26612 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ACSS2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.25 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Effect of Microbial Status on Hepatic Odd-Chain Fatty Acids Is Diet-Dependent.
Acetate Promotes T Cell Effector Function during Glucose Restriction.
Weitkunat K, Bishop CA, Wittmüss M, Machate T, Schifelbein T, Schulze MB, Klaus S
Nutrients 2021 May 4;13(5)
Nutrients 2021 May 4;13(5)
Acetate Promotes T Cell Effector Function during Glucose Restriction.
Qiu J, Villa M, Sanin DE, Buck MD, O'Sullivan D, Ching R, Matsushita M, Grzes KM, Winkler F, Chang CH, Curtis JD, Kyle RL, Van Teijlingen Bakker N, Corrado M, Haessler F, Alfei F, Edwards-Hicks J, Maggi LB Jr, Zehn D, Egawa T, Bengsch B, Klein Geltink RI, Jenuwein T, Pearce EJ, Pearce EL
Cell reports 2019 May 14;27(7):2063-2074.e5
Cell reports 2019 May 14;27(7):2063-2074.e5
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using an ACSS2 polyclonal antibody (Product # PA5-26612) in K562 cell lysates (35 µg per lane).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using an ACSS2 polyclonal antibody (Product # PA5-26612) in mouse liver tissue lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Germ-free mice (GF) and SIHUMI mice (SIH) on CHOW diet display a reduced hepatic de novo fatty acid synthesis, but increased relative OCFA compared to conventional mice (CONV). Twenty-week-old male C3H/HeOuJ mice on CHOW diet (Altromin fortified 1314): ( A ) long-chain fatty acid (LCFA) composition of hepatic phospholipid fraction. Results are expressed as area percentage of individual fatty acids to total area of detected fatty acids. ( B ) Hepatic triglyceride (TG) concentration. Analysis of fatty acid metabolism with both ( C ) mRNA and ( E ) protein expression. ( D ) Representative Western blots for fatty acid signaling. Data are mean + SEM ( n = 6) and expression data were normalized to CONV group. Hprt was used as the housekeeping gene and GAPDH as the loading control for the protein. * p < 0.05; ** p < 0.01; *** p < 0.001.