Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- MAB10077-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TDAG8/GPR65 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human TDAG8/GPR65 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q81IYL9
- Isotype
- IgG
- Antibody clone number
- 976128
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human TDAG8/GPR65 by Western Blot. Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with human TDAG8/GPR65. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TDAG8/GPR65 Monoclonal Antibody (Catalog # MAB10077) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for TDAG8/GPR65 at approximately 85-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TDAG8/GPR65 in THP-1 and K562 Human Cell Lines. TDAG8/GPR65 was detected in immersion fixed THP-1 human acute monocytic leukemia cell line (left panel, positive stain) and K562 human chronic myelogenous leukemia cell line (right panel, negative stain) using Mouse Anti-Human TDAG8/GPR65 Monoclonal Antibody (Catalog # MAB10077) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.