Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AGR-043-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPR65 (TDAG8) (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 50 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 X g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4)brain membranes: - 1,2. Anti-GPR65 (TDAG8) (extracellular) Antibody (#AGR-043), (1:200).3,4. Anti-GPR65 (TDAG8) (extracellular) Antibody , preincubated with GPR65/TDAG8 (extracellular) Blocking Peptide (#BLP-GR043).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human Jurkat T-cell leukemia (lanes 1 and 4), human THP-1 monocytic leukemia (lanes 2 and 5) and human HL-60 promyelocytic leukemia (lanes 3 and 6) cell line lysates: - 1-3. Anti-GPR65 (TDAG8) (extracellular) Antibody (#AGR-043), (1:200).4-6. Anti-GPR65 (TDAG8) (extracellular) Antibody , preincubated with GPR65/TDAG8 (extracellular) Blocking Peptide (#BLP-GR043).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of GPR65 in rat fornix - Immunohistochemical staining of immersion-fixed, free floating rat frozen brain sections using Anti-GPR65 (TDAG8) (extracellular) Antibody (#AGR-043), (1:1200), followed by goat- Anti-rabbit-AlexaFluor-488. GPR65 staining (green) appears in glial processes (vertical arrows) and in the side of fornix (FX) facing the ventricle (V, horizontal arrow). Cell nuclei are stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of GPR65 in live intact human Jurkat T-cell leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-GPR65 (TDAG8) (extracellular) Antibody (#AGR-043), 5 µg + goat- Anti-rabbit-FITC.