PA5-46937

antibody from Invitrogen Antibodies

Targeting: CXCL6 CKA-3, GCP-2, SCYB6

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-46937

Provider

Invitrogen Antibodies

Product name

CXCL6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant mouse (rm) MIP-2, rmKC, rmCXCL6, recombinant human (rh) ENA-78, recombinant rat (rr) CINC-2 alpha, rmCRG-2, rhNAP-2 is observed and less than 5% cross-reactivity with rrCINC-2 beta and rhMCP-3 is observed.

Concentration

0.2 mg/mL

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization of CXCL6 in BaF3 mouse pro‚B cell line transfected with human CXCR2. Samples were incubated in CXCL6 polyclonal antibody (Product # PA5-46937). Recombinant Human CXCL6/GCP‚2 chemoattracts the BaF3 mouse pro‚B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CXCL6/GCP‚2 (80 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CXCL6/GCP‚2 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.8-4.0 µg/mL.