Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF333 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CXCL6/GCP-2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human CXCL6/GCP-2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) MIP-2, rmKC, recombinant rat (rr) CINC-2 alpha , rmCRG-2, recombinant human (rh) NAP-2, rrCINC-2 beta and rhMCP-3 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P80162
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Chemotaxis Induced by CXCL6/GCP-2 and Neutralization by Human CXCL6/GCP-2 Antibody. Recombinant Human CXCL6/ GCP-2 (Catalog # 333-GC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL6/ GCP-2 (80 ng/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Human CXCL6/GCP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF333). The ND50 is typically 0.8-4.0 µg/mL.