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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB333 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CXCL6/GCP-2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human CXCL6/GCP-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.04% cross-reactivity with recombinant human (rh) NAP-2, rhGRO alpha , rhGRO beta , rhBLC, rhENA-78, recombinant mouse (rm) CXCL6, rmMIP-2, rhIL-8, rhIP-10, rhMIG, rhSDF-1 alpha , and rhSDF-1 beta is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P80162- Isotype
- IgG
- Antibody clone number
- 60910
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Metronomic chemotherapy prevents therapy-induced stromal activation and induction of tumor-initiating cells.
GCP-2/CXCL6 synergizes with other endothelial cell-derived chemokines in neutrophil mobilization and is associated with angiogenesis in gastrointestinal tumors.
Chan TS, Hsu CC, Pai VC, Liao WY, Huang SS, Tan KT, Yen CJ, Hsu SC, Chen WY, Shan YS, Li CR, Lee MT, Jiang KY, Chu JM, Lien GS, Weaver VM, Tsai KK
The Journal of experimental medicine 2016 Dec 12;213(13):2967-2988
The Journal of experimental medicine 2016 Dec 12;213(13):2967-2988
GCP-2/CXCL6 synergizes with other endothelial cell-derived chemokines in neutrophil mobilization and is associated with angiogenesis in gastrointestinal tumors.
Gijsbers K, Gouwy M, Struyf S, Wuyts A, Proost P, Opdenakker G, Penninckx F, Ectors N, Geboes K, Van Damme J
Experimental cell research 2005 Feb 15;303(2):331-42
Experimental cell research 2005 Feb 15;303(2):331-42
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Chemotaxis Induced by CXCL6/GCP-2 and Neutral-ization by Human CXCL6/ GCP-2 Antibody. Recombinant Human CXCL6/ GCP-2 (Catalog # 333-GC) induces chemotaxis in the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Under these conditions, chemotaxis elicited by CXCL6/GCP-2 is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL6/GCP-2 Monoclonal Antibody (Catalog # MAB333). The ND50 is typically 8-30 μg/mL.
