NBP1-82876

antibody from Novus Biologicals

Targeting: KIF22 Kid, KNSL4, OBP-1, OBP-2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-82876

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-82876, RRID:AB_11037073

Product name

Rabbit Polyclonal KIF22 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human KIF22 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.

Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg E
Nature methods 2013 Apr;10(4):315-23

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: KIF22 Antibody [NBP1-82876] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: KIF22 Antibody [NBP1-82876] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: KIF22 Antibody [NBP1-82876] - Staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules.