LS-C675572

antibody from LSBio

Targeting: COPS3 CSN3, SGN3

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C675572

Provider

LSBio

Product name

COPS3 / CSN3 Antibody LS-C675572

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot All Lanes: COPS3antibody at 3.41ug/ml Lane 1 : MCF7 whole cell lysate Lane 2 : Hela whole cell lysate Lane 3 : 293T whole cell lysate Lane 4 : HT29 whole cell lysate Lane 5 : Mouse heart tissue Lane 6 : Mouse brain tissue Lane 7 : Mouse ovarian tissue Secondary Goat polyclonal to Rabbit IgG at 1/10000 dilution Predicted band size: 48,46 kDa Observed band size: 45 kDa

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating COPS3 in HeLa whole cell lysate Lane 1: Rabbit monoclonal IgG(1ug)instead of product in HeLa whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG,specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: product(4ug)+ HeLa whole cell lysate(500ug) Lane 3: HeLa whole cell lysate (20ug)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating COPS3 in HeLa whole cell lysate Lane 1: Rabbit monoclonal IgG(1ug)instead of product in HeLa whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG,specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: product(4ug)+ HeLa whole cell lysate(500ug) Lane 3: HeLa whole cell lysate (20ug)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating COPS3 in HeLa whole cell lysate Lane 1: Rabbit monoclonal IgG(1ug)instead of product in HeLa whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG,specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: product(4ug)+ HeLa whole cell lysate(500ug) Lane 3: HeLa whole cell lysate (20ug)