Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [7]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-34854 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- COPS3 Recombinant Rabbit Monoclonal Antibody (JE47-48)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: Human skeletal muscle tissue, Rat bone marrow, A431, HCT116, rat heart tissue, human liver cancer tissue, human breast tissue, human pancreas tissue, mouse kidney tissue, mouse heart tissue, 293.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JE47-48
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of COPS3/CSN3 in Positive control: Lane 1: Human skeletal muscle tissue lysate, Lane 2: Rat bone marrow lysate. Samples were transferred to PVDF membrane, blocked with 5% BSA (1 hour), incubated with COPS3/CSN3 monoclonal antibody (Product # MA5-34854), at a dilution of 1:500, followed by Goat Anti-Rabbit IgG-HRP (1 hour) with a dilution of 1:5000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of COPS3/CSN3 in HCT116 cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with COPS3/CSN3 monoclonal antibody (Product # MA5-34854) at a dilution of 1:500 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of COPS3/CSN3 in A431 cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with COPS3/CSN3 monoclonal antibody (Product # MA5-34854) at a dilution of 1:500 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of COPS3/CSN3 in paraffin-embedded human breast tissue. Samples were heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, 20 min) and blocked in 5% BSA (30 min, room temperature), incubated with COPS3/CSN3 monoclonal antibody (Product # MA5-34854) at a dilution of 1:50 (30 min, room temperature), and followed by HRP conjugated compact polymer system, DAB, counterstained with hematoxylin (mounted with DPX).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of COPS3/CSN3 in paraffin-embedded rat heart tissue. Samples were heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, 20 min) and blocked in 5% BSA (30 min, room temperature), incubated with COPS3/CSN3 monoclonal antibody (Product # MA5-34854) at a dilution of 1:200 (30 min, room temperature), and followed by HRP conjugated compact polymer system, DAB, counterstained with hematoxylin (mounted with DPX).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of COPS3/CSN3 in paraffin-embedded mouse kidney tissue. Samples were heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, 20 min) and blocked in 5% BSA (30 min, room temperature), incubated with COPS3/CSN3 monoclonal antibody (Product # MA5-34854) at a dilution of 1:50 (30 min, room temperature), and followed by HRP conjugated compact polymer system, DAB, counterstained with hematoxylin (mounted with DPX).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of COPS3/CSN3 in paraffin-embedded mouse heart tissue. Samples were heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, 20 min) and blocked in 5% BSA (30 min, room temperature), incubated with COPS3/CSN3 monoclonal antibody (Product # MA5-34854) at a dilution of 1:50 (30 min, room temperature), and followed by HRP conjugated compact polymer system, DAB, counterstained with hematoxylin (mounted with DPX).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of COPS3/CSN3 in paraffin-embedded human pancreas tissue. Samples were heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, 20 min) and blocked in 5% BSA (30 min, room temperature), incubated with COPS3/CSN3 monoclonal antibody (Product # MA5-34854) at a dilution of 1:50 (30 min, room temperature), and followed by HRP conjugated compact polymer system, DAB, counterstained with hematoxylin (mounted with DPX).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of COPS3/CSN3 in paraffin-embedded human liver cancer tissue. Samples were heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, 20 min) and blocked in 5% BSA (30 min, room temperature), incubated with COPS3/CSN3 monoclonal antibody (Product # MA5-34854) at a dilution of 1:50 (30 min, room temperature), and followed by HRP conjugated compact polymer system, DAB, counterstained with hematoxylin (mounted with DPX).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of COPS3/CSN3 in paraffin-embedded human breast tissue. Samples were heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, 20 min) and blocked in 5% BSA (30 min, room temperature), incubated with COPS3/CSN3 monoclonal antibody (Product # MA5-34854) at a dilution of 1:50 (30 min, room temperature), and followed by HRP conjugated compact polymer system, DAB, counterstained with hematoxylin (mounted with DPX).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of COPS3/CSN3 in 293 cells, unlabelled sample (control; cells without incubation with primary antibody; black). Samples were incubated with COPS3/CSN3 monoclonal antibody (Product # MA5-34854) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG with a dilution of 1:500 (30 min).
