Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB433-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse LIX Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse LIX in Western blots. In Western blots, no cross-reactivity with recombinant human CXCL1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12/SDF-1 alpha , 12/SDF-1 beta , 13, recombinant mouse CXCL1, 2, 6, 9, 10, 12/SDF-1 alpha , 13, recombinant rat CXCL1, 3/CINC-2 alpha , 3/CINC-2 beta , or recombinant porcine CXCL8 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P50228
- Isotype
- IgG
- Antibody clone number
- 61905
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Pulmonary Epithelial TLR4 Activation Leads to Lung Injury in Neonatal Necrotizing Enterocolitis.
Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections.
Castration-induced changes in mouse epididymal white adipose tissue.
Matrix metalloproteinase-8 deficiency promotes granulocytic allergen-induced airway inflammation.
Circulating CXC-chemokine concentrations in a murine intestinal ischemia-reperfusion model.
Transcriptional profiling of lipopolysaccharide-induced acute lung injury.
Jia H, Sodhi CP, Yamaguchi Y, Lu P, Martin LY, Good M, Zhou Q, Sung J, Fulton WB, Nino DF, Prindle T Jr, Ozolek JA, Hackam DJ
Journal of immunology (Baltimore, Md. : 1950) 2016 Aug 1;197(3):859-71
Journal of immunology (Baltimore, Md. : 1950) 2016 Aug 1;197(3):859-71
Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections.
Junkins RD, MacNeil AJ, Wu Z, McCormick C, Lin TJ
Journal of immunology (Baltimore, Md. : 1950) 2013 May 15;190(10):5178-86
Journal of immunology (Baltimore, Md. : 1950) 2013 May 15;190(10):5178-86
Castration-induced changes in mouse epididymal white adipose tissue.
Floryk D, Kurosaka S, Tanimoto R, Yang G, Goltsov A, Park S, Thompson TC
Molecular and cellular endocrinology 2011 Oct 15;345(1-2):58-67
Molecular and cellular endocrinology 2011 Oct 15;345(1-2):58-67
Matrix metalloproteinase-8 deficiency promotes granulocytic allergen-induced airway inflammation.
Gueders MM, Balbin M, Rocks N, Foidart JM, Gosset P, Louis R, Shapiro S, Lopez-Otin C, Noël A, Cataldo DD
Journal of immunology (Baltimore, Md. : 1950) 2005 Aug 15;175(4):2589-97
Journal of immunology (Baltimore, Md. : 1950) 2005 Aug 15;175(4):2589-97
Circulating CXC-chemokine concentrations in a murine intestinal ischemia-reperfusion model.
Maheshwari A, Christensen RD, Calhoun DA, Dimmitt RA, Lacson A
Fetal and pediatric pathology 2004 Mar-Jun;23(2-3):145-57
Fetal and pediatric pathology 2004 Mar-Jun;23(2-3):145-57
Transcriptional profiling of lipopolysaccharide-induced acute lung injury.
Jeyaseelan S, Chu HW, Young SK, Worthen GS
Infection and immunity 2004 Dec;72(12):7247-56
Infection and immunity 2004 Dec;72(12):7247-56
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by LIX and Neutralization by Mouse LIX Antibody. Recombinant Mouse LIX (Catalog # 433-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse LIX (0.2 µg/mL) is neutralized (green line) by increasing concen-trations of Rat Anti-Mouse LIX Monoclonal Antibody (Catalog # MAB433). The ND50 is typically 0.5-2.5 µg/mL.