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- Antibody Data
- Antigen structure
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- Other assay [3]
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- Product number
- 710010 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CXCL5 Recombinant Polyclonal Antibody (9HCLC)
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 9HCLC
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CXCL5 was immunoprecipitated using 5 µg of the CXCL5 Recombinant Rabbit Polyclonal Antibody (Product # 710010) from lysate of A549 cells treated with PMA (200 nM/20 minutes) (Lane 3) using the Dynabeads® Protein A Immunoprecipitation Kit (Product # 10006D). Normal Rabbit IgG was used as a Isotype control (Lane 2). 10 % input represents the cell extract used for immunoprecipitation (Lane 1). Western blot analysis was performed using CXCL5 Recombinant Rabbit Polyclonal Antibody (Product # 710010) and Goat anti-Rabbit IgG Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CXCL5 was immunoprecipitated using 5 µg of the CXCL5 Recombinant Rabbit Polyclonal Antibody (Product # 710010) from lysate of A549 cells treated with PMA (200 nM/20 minutes) (Lane 3) using the Dynabeads® Protein A Immunoprecipitation Kit (Product # 10006D). Normal Rabbit IgG was used as a Isotype control (Lane 2). 10 % input represents the cell extract used for immunoprecipitation (Lane 1). Western blot analysis was performed using CXCL5 Recombinant Rabbit Polyclonal Antibody (Product # 710010) and Goat anti-Rabbit IgG Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CXCL5 was immunoprecipitated using 5 æg of the CXCL5 Recombinant Rabbit Polyclonal Antibody (Product # 710010) from lysate of A549 cells treated with PMA (200 nM/20 minutes) (Lane 3) using the Dynabeads® Protein A Immunoprecipitation Kit (Product # 10006D). Normal Rabbit IgG was used as a Isotype control (Lane 2). 10 % input represents the cell extract used for immunoprecipitation (Lane 1). Western blot analysis was performed using CXCL5 Recombinant Rabbit Polyclonal Antibody (Product # 710010) and Goat anti-Rabbit IgG Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 æg/mL, 1:2500 dilution). Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).
