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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- AF4480 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal LRPAP Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse LRPAP in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human LRPAP is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Low-density lipoprotein receptor represents an apolipoprotein E-independent pathway of Aβ uptake and degradation by astrocytes.
Basak JM, Verghese PB, Yoon H, Kim J, Holtzman DM
The Journal of biological chemistry 2012 Apr 20;287(17):13959-71
The Journal of biological chemistry 2012 Apr 20;287(17):13959-71
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Mouse LRPAP by Western Blot. Western blot shows lysates of D3 mouse embryonic stem cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse LRPAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4480) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LRPAP at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
