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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [7]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-27986 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RAP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa, HepG2, HCT116, U87-MG, SK-N-SH, IMR32, SK-N-AS.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RAP using 30 µg of A) HeLa and B) HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a RAP polyclonal antibody (Product # PA5-27986) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RAP in Various tissue extracts (50 µg). Samples were separated by 10% SDS-PAGE and the membrane was probed with RAP Polyclonal antibody (Product # PA5-27986) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RAP in Various whole cell extracts (30 µg). Samples were separated by 10% SDS-PAGE and the membrane was probed with RAP Polyclonal antibody (Product # PA5-27986) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RAP Polyclonal Antibody (Product # PA5-27986). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with RAP antibody [N1C3] RAP Polyclonal Antibody (Product # PA5-27986) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RAP was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a RAP Polyclonal Antibody (Product # PA5-27986) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RAP Polyclonal Antibody (Product # PA5-27986). Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with RAP antibody [N1C3] RAP Polyclonal Antibody (Product # PA5-27986) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RAP Polyclonal Antibody (Product # PA5-27986). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with RAP antibody [N1C3] RAP Polyclonal Antibody (Product # PA5-27986) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of RAP was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: RAP Polyclonal Antibody (Product # PA5-27986) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of RAP was performed in paraffin-embedded rat brain tissue using RAP Polyclonal Antibody (Product # PA5-27986) at a dilution of 1:500.
