PA5-27986

antibody from Invitrogen Antibodies

Targeting: LRPAP1 A2MRAP, HBP44, RAP

Antibody data

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Validation data

Product number: PA5-27986 - Provider product page
Provider: Invitrogen Antibodies
Product name: RAP Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: A549, HeLa, HepG2, HCT116, U87-MG, SK-N-SH, IMR32, SK-N-AS. Predicted reactivity: Chimpanzee (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

LRPAP1 protein structure - PA5-27986 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of RAP using 30 µg of A) HeLa and B) HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a RAP polyclonal antibody (Product # PA5-27986) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of RAP in Various tissue extracts (50 µg). Samples were separated by 10% SDS-PAGE and the membrane was probed with RAP Polyclonal antibody (Product # PA5-27986) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of RAP in Various whole cell extracts (30 µg). Samples were separated by 10% SDS-PAGE and the membrane was probed with RAP Polyclonal antibody (Product # PA5-27986) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using RAP Polyclonal Antibody (Product # PA5-27986). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with RAP antibody [N1C3] RAP Polyclonal Antibody (Product # PA5-27986) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of RAP was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a RAP Polyclonal Antibody (Product # PA5-27986) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using RAP Polyclonal Antibody (Product # PA5-27986). Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with RAP antibody [N1C3] RAP Polyclonal Antibody (Product # PA5-27986) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using RAP Polyclonal Antibody (Product # PA5-27986). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with RAP antibody [N1C3] RAP Polyclonal Antibody (Product # PA5-27986) diluted at 1:1,000.