LS-C797243

antibody from LSBio

Targeting: DAPK2 DRP-1, MGC119312

Western blot

Immunocytochemistry

Antibody data

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Validation data

Product number: LS-C797243 - Provider product page
Provider: LSBio
Product name: DAPK2 / DAP Kinase 2 Antibody (clone 1B5, Carrier-free) LS-C797243
Antibody type: Monoclonal
Description: Purified from ascites.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 1B5
Storage: Store at -20°C. Avoid freeze-thaw cycles.

DAPK2 protein structure - LS-C797243 shown in red.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Immunoprecipitation(IP) of DAPK2 by using monoclonal anti-DAPK2 antibodies (Negative control: IP without adding anti-DAPK2 antibody.). For each experiment, 500ul of DDK tagged DAPK2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-DAPK2 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Immunoprecipitation(IP) of DAPK2 by using monoclonal anti-DAPK2 antibodies (Negative control: IP without adding anti-DAPK2 antibody.). For each experiment, 500ul of DDK tagged DAPK2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-DAPK2 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Immunoprecipitation(IP) of DAPK2 by using monoclonal anti-DAPK2 antibodies (Negative control: IP without adding anti-DAPK2 antibody.). For each experiment, 500ul of DDK tagged DAPK2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-DAPK2 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.