NBP2-02477

antibody from Novus Biologicals

Targeting: DAPK2 DRP-1, MGC119312

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-02477

Provider

Novus Biologicals

Product name

Mouse Monoclonal DAP Kinase 2 Antibody

Antibody type

Monoclonal

Description

Affinity purified.

Reactivity

Human, Rat, Canine, Simian

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

0.76 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-DAP Kinase 2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAP Kinase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DAP Kinase 2.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DAP Kinase 2 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-DAP Kinase 2 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of paraffin-embedded Human colon tissue using anti-DAP Kinase 2 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of paraffin-embedded Human Kidney tissue using anti-DAP Kinase 2 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of paraffin-embedded Human liver tissue using anti-DAP Kinase 2 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of paraffin-embedded Human lung tissue using anti-DAP Kinase 2 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of paraffin-embedded Human pancreas tissue using anti-DAP Kinase 2 mouse monoclonal antibody.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DAP Kinase 2 antibody, and then analyzed by flow cytometry.