PA1-41305

antibody from Invitrogen Antibodies

Targeting: DAPK2 DRP-1, MGC119312

Western blot (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-41305

Provider

Invitrogen Antibodies

Product name

DAPK2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Suggested positive control: antigen standard for DAPK2 (transient overexpression lysate), rat kidney tissue, rat kidney protein.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 μg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

DAPK1 loss triggers tumor invasion in colorectal tumor cells.

Steinmann S, Kunze P, Hampel C, Eckstein M, Bertram Bramsen J, Muenzner JK, Carlé B, Ndreshkjana B, Kemenes S, Gasparini P, Friedrich O, Andersen C, Geppert C, Wang S, Eyupoglu I, Bäuerle T, Hartmann A, Schneider-Stock R
Cell death & disease 2019 Nov 26;10(12):895

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Validation of CRISPR/Cas9-mediated DAPK1 knockout in HCT116 colorectal cancer cells. a Western Blot of DAPK1 expression status in various colorectal cancer cell lines. Representative images of two independent experiments are shown. GAPDH served as loading control. b Immunofluorescence staining of DAPK1 (green) in parental HCT116 cells and DAPK1 ko clones. Cells were counterstained with phalloidin for F-actin (red) and nuclear Hoechst (blue). Fluorescence microscopy was performed using a x 100 oil immersion objective. Representative images of two independent experiments are shown. Scale bar = 20 µm. c Protein expression of DAPK1 family members DAPK1, DAPK2, DAPK3, DRAK1, DRAK2 and DAPK1 phosphorylation target pMLC in HCT116 wildtype cells and DAPK1 ko clones were detected by Western Blotting using specific primary antibodies. Representative images of two independent experiments are shown. * images were cropped here; all samples were analyzed on the same SDS-PAGE gel; ** GAPDH blot has been used twice see Fig.5b, proteins have been loaded on the same membrane. d Western Blot analysis of stem cell markers (CD133, CD44) and EMT markers (epithelial marker: E-cad = E-cadherin, mesenchymal marker: Vimentin). Representative images of two independent experiments are shown.*images were cropped here; all samples were analyzed on the same SDS-PAGE gel. e Representative images of endogenous phospho-ERK1/2 (red) levels of immunostained HCT116 cells and DAPK1 ko clones examined by