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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF517 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal CXCL3/GRO gamma/CINC-2/DCIP-1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects rat CXCL3/CINC-2 alpha/beta in direct ELISAs and Western blots. In direct ELISAs, almost 100% cross-reactivity with recombinant rat (rr) CINC-2 alpha is observed, approximately 50% cross-reactivity with recombinant mouse (rm) MIP-2 is observed, 35% cross-reactivity with rmKC is observed, 15% cross-reactivity with rrCINC-1 is observed, and 10% cross-reactivity with recombinant human (rh) IL-8, rhGRO alpha , rhGRO beta , and rhGRO gamma is observed.
- Reactivity
- Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Chemotaxis Induced by CXCL3/CINC-2 beta and Neutral-ization by Rat CXCL3/ CINC-2 alpha/beta Antibody. Recombinant Rat CXCL3/ CINC-2 beta (Catalog # 517-CB) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL3/ CINC-2 beta (0.05 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CXCL3/ CINC-2 alpha/beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF517). The ND50 is typically 0.4-2.0 µg/mL.
