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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF5568 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CXCL3/GRO gamma/CINC-2/DCIP-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse CXCL3/GRO gamma/CINC-2/DCIP-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant rat CINC-2 is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
AAI17017- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Chemotaxis Induced by CXCL3/DCIP-1 and Neutral-ization by Mouse CXCL3/ DCIP-1 Antibody. Recombinant Mouse CXCL3/DCIP-1 (Catalog # 5568-CA) chemo-attracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL3/ DCIP-1 (100 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Mouse CXCL3/ DCIP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5568). The ND50 is typically 0.5-3 µg/mL.
