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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [33]
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- Blocking/Neutralizing [1]
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- Product number
- MAB452-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CXCL2/GRO beta/MIP-2/CINC-3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse CXCL2 in ELISAs and Western blots. In ELISAs, does not cross-react with recombinant human (rh) CXCL2/GRO beta , rhCXCL3/GRO gamma , or recombinant mouse CXCL1/KC.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P10889- Isotype
- IgG
- Antibody clone number
- 40605
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Modulation of double-stranded RNA pattern recognition receptor signaling in ovarian cancer cells promotes inflammatory queues.
IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3.
Mucin 1 protects against severe Streptococcus pneumoniae infection.
Malondialdehyde epitopes are sterile mediators of hepatic inflammation in hypercholesterolemic mice.
Absence of DCIR1 reduces the mortality rate of endotoxemic hepatitis in mice.
Patrolling monocytes promote intravascular neutrophil activation and glomerular injury in the acutely inflamed glomerulus.
Pulmonary Epithelial Cell-Derived Cytokine TGF-β1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function.
Mesenchymal Stem Cells Ameliorate Atherosclerotic Lesions via Restoring Endothelial Function.
Syndecan-1 in the mouse parietal peritoneum microcirculation in inflammation.
G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis.
Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections.
Activation of MDL-1 (CLEC5A) on immature myeloid cells triggers lethal shock in mice.
A chemotactic gradient sequestered on endothelial heparan sulfate induces directional intraluminal crawling of neutrophils.
Intrinsic renal cell and leukocyte-derived TLR4 aggravate experimental anti-MPO glomerulonephritis.
Lipid-cytokine-chemokine cascade drives neutrophil recruitment in a murine model of inflammatory arthritis.
Chlamydial respiratory infection during allergen sensitization drives neutrophilic allergic airways disease.
Increased cytokine production in interleukin-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling.
CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
Neutrophil chemokines KC and macrophage-inflammatory protein-2 are newly synthesized by tissue macrophages using distinct TLR signaling pathways.
Critical role of CD44 in hepatotoxin-mediated liver injury.
The coordinated action of G-CSF and ELR + CXC chemokines in neutrophil mobilization during acute inflammation.
HIF-1alpha regulates epithelial inflammation by cell autonomous NFkappaB activation and paracrine stromal remodeling.
Type II NKT cell-mediated anergy induction in type I NKT cells prevents inflammatory liver disease.
Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury.
Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors.
Overexpression of suppressor of cytokine signaling-5 in T cells augments innate immunity during septic peritonitis.
Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4).
Blockade of experimental atopic dermatitis via topical NF-kappaB decoy oligonucleotide.
Sequential ELISA to profile multiple cytokines from small volumes.
Matrix metalloproteinase-8 deficiency promotes granulocytic allergen-induced airway inflammation.
Transcriptional profiling of lipopolysaccharide-induced acute lung injury.
Endogenous IL-17 as a mediator of neutrophil recruitment caused by endotoxin exposure in mouse airways.
Rapid up-regulation of CXC chemokines in the airways after Ag-specific CD4+ T cell activation.
Muccioli M, Nandigam H, Loftus T, Singh M, Venkatesh A, Wright J, Pate M, McCall K, Benencia F
Oncotarget 2018 Nov 30;9(94):36666-36683
Oncotarget 2018 Nov 30;9(94):36666-36683
IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3.
Lv R, Zhao J, Lei M, Xiao D, Yu Y, Xie J
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;42(5):1961-1972
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;42(5):1961-1972
Mucin 1 protects against severe Streptococcus pneumoniae infection.
Dhar P, Ng GZ, Dunne EM, Sutton P
Virulence 2017 Nov 17;8(8):1631-1642
Virulence 2017 Nov 17;8(8):1631-1642
Malondialdehyde epitopes are sterile mediators of hepatic inflammation in hypercholesterolemic mice.
Busch CJ, Hendrikx T, Weismann D, Jäckel S, Walenbergh SM, Rendeiro AF, Weißer J, Puhm F, Hladik A, Göderle L, Papac-Milicevic N, Haas G, Millischer V, Subramaniam S, Knapp S, Bennett KL, Bock C, Reinhardt C, Shiri-Sverdlov R, Binder CJ
Hepatology (Baltimore, Md.) 2017 Apr;65(4):1181-1195
Hepatology (Baltimore, Md.) 2017 Apr;65(4):1181-1195
Absence of DCIR1 reduces the mortality rate of endotoxemic hepatitis in mice.
Ishiguro T, Fukawa T, Akaki K, Nagaoka K, Takeda T, Iwakura Y, Inaba K, Takahara K
European journal of immunology 2017 Apr;47(4):704-712
European journal of immunology 2017 Apr;47(4):704-712
Patrolling monocytes promote intravascular neutrophil activation and glomerular injury in the acutely inflamed glomerulus.
Finsterbusch M, Hall P, Li A, Devi S, Westhorpe CL, Kitching AR, Hickey MJ
Proceedings of the National Academy of Sciences of the United States of America 2016 Aug 30;113(35):E5172-81
Proceedings of the National Academy of Sciences of the United States of America 2016 Aug 30;113(35):E5172-81
Pulmonary Epithelial Cell-Derived Cytokine TGF-β1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function.
Denney L, Byrne AJ, Shea TJ, Buckley JS, Pease JE, Herledan GM, Walker SA, Gregory LG, Lloyd CM
Immunity 2015 Nov 17;43(5):945-58
Immunity 2015 Nov 17;43(5):945-58
Mesenchymal Stem Cells Ameliorate Atherosclerotic Lesions via Restoring Endothelial Function.
Lin YL, Yet SF, Hsu YT, Wang GJ, Hung SC
Stem cells translational medicine 2015 Jan;4(1):44-55
Stem cells translational medicine 2015 Jan;4(1):44-55
Syndecan-1 in the mouse parietal peritoneum microcirculation in inflammation.
Kowalewska PM, Patrick AL, Fox-Robichaud AE
PloS one 2014;9(9):e104537
PloS one 2014;9(9):e104537
G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis.
Raghuwanshi SK, Smith N, Rivers EJ, Thomas AJ, Sutton N, Hu Y, Mukhopadhyay S, Chen XL, Leung T, Richardson RM
Journal of immunology (Baltimore, Md. : 1950) 2013 May 15;190(10):5329-36
Journal of immunology (Baltimore, Md. : 1950) 2013 May 15;190(10):5329-36
Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections.
Junkins RD, MacNeil AJ, Wu Z, McCormick C, Lin TJ
Journal of immunology (Baltimore, Md. : 1950) 2013 May 15;190(10):5178-86
Journal of immunology (Baltimore, Md. : 1950) 2013 May 15;190(10):5178-86
Activation of MDL-1 (CLEC5A) on immature myeloid cells triggers lethal shock in mice.
Cheung R, Shen F, Phillips JH, McGeachy MJ, Cua DJ, Heyworth PG, Pierce RH
The Journal of clinical investigation 2011 Nov;121(11):4446-61
The Journal of clinical investigation 2011 Nov;121(11):4446-61
A chemotactic gradient sequestered on endothelial heparan sulfate induces directional intraluminal crawling of neutrophils.
Massena S, Christoffersson G, Hjertström E, Zcharia E, Vlodavsky I, Ausmees N, Rolny C, Li JP, Phillipson M
Blood 2010 Sep 16;116(11):1924-31
Blood 2010 Sep 16;116(11):1924-31
Intrinsic renal cell and leukocyte-derived TLR4 aggravate experimental anti-MPO glomerulonephritis.
Summers SA, van der Veen BS, O'Sullivan KM, Gan PY, Ooi JD, Heeringa P, Satchell SC, Mathieson PW, Saleem MA, Visvanathan K, Holdsworth SR, Kitching AR
Kidney international 2010 Dec;78(12):1263-74
Kidney international 2010 Dec;78(12):1263-74
Lipid-cytokine-chemokine cascade drives neutrophil recruitment in a murine model of inflammatory arthritis.
Chou RC, Kim ND, Sadik CD, Seung E, Lan Y, Byrne MH, Haribabu B, Iwakura Y, Luster AD
Immunity 2010 Aug 27;33(2):266-78
Immunity 2010 Aug 27;33(2):266-78
Chlamydial respiratory infection during allergen sensitization drives neutrophilic allergic airways disease.
Horvat JC, Starkey MR, Kim RY, Beagley KW, Preston JA, Gibson PG, Foster PS, Hansbro PM
Journal of immunology (Baltimore, Md. : 1950) 2010 Apr 15;184(8):4159-69
Journal of immunology (Baltimore, Md. : 1950) 2010 Apr 15;184(8):4159-69
Increased cytokine production in interleukin-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling.
Nold-Petry CA, Nold MF, Nielsen JW, Bustamante A, Zepp JA, Storm KA, Hong JW, Kim SH, Dinarello CA
The Journal of biological chemistry 2009 Sep 18;284(38):25900-11
The Journal of biological chemistry 2009 Sep 18;284(38):25900-11
CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T
Immunology 2008 Oct;125(2):272-9
Immunology 2008 Oct;125(2):272-9
Neutrophil chemokines KC and macrophage-inflammatory protein-2 are newly synthesized by tissue macrophages using distinct TLR signaling pathways.
De Filippo K, Henderson RB, Laschinger M, Hogg N
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 15;180(6):4308-15
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 15;180(6):4308-15
Critical role of CD44 in hepatotoxin-mediated liver injury.
Kimura K, Nagaki M, Kakimi K, Saio M, Saeki T, Okuda Y, Kuwata K, Moriwaki H
Journal of hepatology 2008 Jun;48(6):952-61
Journal of hepatology 2008 Jun;48(6):952-61
The coordinated action of G-CSF and ELR + CXC chemokines in neutrophil mobilization during acute inflammation.
Wengner AM, Pitchford SC, Furze RC, Rankin SM
Blood 2008 Jan 1;111(1):42-9
Blood 2008 Jan 1;111(1):42-9
HIF-1alpha regulates epithelial inflammation by cell autonomous NFkappaB activation and paracrine stromal remodeling.
Scortegagna M, Cataisson C, Martin RJ, Hicklin DJ, Schreiber RD, Yuspa SH, Arbeit JM
Blood 2008 Apr 1;111(7):3343-54
Blood 2008 Apr 1;111(7):3343-54
Type II NKT cell-mediated anergy induction in type I NKT cells prevents inflammatory liver disease.
Halder RC, Aguilera C, Maricic I, Kumar V
The Journal of clinical investigation 2007 Aug;117(8):2302-12
The Journal of clinical investigation 2007 Aug;117(8):2302-12
Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury.
Gao H, Hoesel LM, Guo RF, Rancilio NJ, Sarma JV, Ward PA
Journal of immunology (Baltimore, Md. : 1950) 2006 Jul 1;177(1):612-20
Journal of immunology (Baltimore, Md. : 1950) 2006 Jul 1;177(1):612-20
Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors.
Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjöstedt A, Edebro H, Forsman M, Byström M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK
Infection and immunity 2006 Dec;74(12):6730-8
Infection and immunity 2006 Dec;74(12):6730-8
Overexpression of suppressor of cytokine signaling-5 in T cells augments innate immunity during septic peritonitis.
Watanabe H, Kubo M, Numata K, Takagi K, Mizuta H, Okada S, Ito T, Matsukawa A
Journal of immunology (Baltimore, Md. : 1950) 2006 Dec 15;177(12):8650-7
Journal of immunology (Baltimore, Md. : 1950) 2006 Dec 15;177(12):8650-7
Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4).
Ramos CD, Fernandes KS, Canetti C, Teixeira MM, Silva JS, Cunha FQ
European journal of immunology 2006 Aug;36(8):2025-34
European journal of immunology 2006 Aug;36(8):2025-34
Blockade of experimental atopic dermatitis via topical NF-kappaB decoy oligonucleotide.
Dajee M, Muchamuel T, Schryver B, Oo A, Alleman-Sposeto J, De Vry CG, Prasad S, Ruhrmund D, Shyamsundar R, Mutnick D, Mai K, Le T, Parham C, Zhang J, Komuves L, Colby T, Hudak S, McEvoy LM, Ehrhardt RO
The Journal of investigative dermatology 2006 Aug;126(8):1792-803
The Journal of investigative dermatology 2006 Aug;126(8):1792-803
Sequential ELISA to profile multiple cytokines from small volumes.
Osuchowski MF, Siddiqui J, Copeland S, Remick DG
Journal of immunological methods 2005 Jul;302(1-2):172-81
Journal of immunological methods 2005 Jul;302(1-2):172-81
Matrix metalloproteinase-8 deficiency promotes granulocytic allergen-induced airway inflammation.
Gueders MM, Balbin M, Rocks N, Foidart JM, Gosset P, Louis R, Shapiro S, Lopez-Otin C, Noël A, Cataldo DD
Journal of immunology (Baltimore, Md. : 1950) 2005 Aug 15;175(4):2589-97
Journal of immunology (Baltimore, Md. : 1950) 2005 Aug 15;175(4):2589-97
Transcriptional profiling of lipopolysaccharide-induced acute lung injury.
Jeyaseelan S, Chu HW, Young SK, Worthen GS
Infection and immunity 2004 Dec;72(12):7247-56
Infection and immunity 2004 Dec;72(12):7247-56
Endogenous IL-17 as a mediator of neutrophil recruitment caused by endotoxin exposure in mouse airways.
Miyamoto M, Prause O, Sjöstrand M, Laan M, Lötvall J, Lindén A
Journal of immunology (Baltimore, Md. : 1950) 2003 May 1;170(9):4665-72
Journal of immunology (Baltimore, Md. : 1950) 2003 May 1;170(9):4665-72
Rapid up-regulation of CXC chemokines in the airways after Ag-specific CD4+ T cell activation.
Knott PG, Gater PR, Dunford PJ, Fuentes ME, Bertrand CP
Journal of immunology (Baltimore, Md. : 1950) 2001 Jan 15;166(2):1233-40
Journal of immunology (Baltimore, Md. : 1950) 2001 Jan 15;166(2):1233-40
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CXCL2/MIP-2 and Neutralization by Mouse CXCL2/MIP-2 Antibody. Recombinant Mouse CXCL2/MIP-2 (Catalog # 452-M2) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL2/MIP-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL2/MIP-2 Monoclonal Antibody (Catalog # MAB452). The ND50 is typically 1-4 µg/mL.
