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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF525 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat CXCL2/GRO beta/MIP-2/CINC-3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects rat CXCL2/GRO beta/MIP-2/CINC-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant rat (rr) CINC-2 alpha is observed, 15% cross-reactivity with rrCINC-2 beta , recombinant human (rh) GRO alpha , rhGRO beta , rhGRO gamma is observed and 5% cross-reactivity with rrCINC-1 is observed.
- Reactivity
- Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P30348- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Rat coronaviruses infect rat alveolar type I epithelial cells and induce expression of CXC chemokines.
Miura TA, Wang J, Holmes KV, Mason RJ
Virology 2007 Dec 20;369(2):288-98
Virology 2007 Dec 20;369(2):288-98
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Chemotaxis Induced by CXCL2/CINC-3 and Neutralization by Rat CXCL2/CINC-3 Antibody. Recombinant Rat CXCL2/CINC-3 (Catalog # 525-C3) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL2/CINC-3 (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Rat CXCL2/CINC-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF525). The ND50 is typically 0.5-2.5 µg/mL.
