PA5-80529

antibody from Invitrogen Antibodies

Targeting: CXCL2 CINC-2a, GRO2, GROb, MGSA-b, MIP-2a, SCYB2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

PA5-80529

Provider

Invitrogen Antibodies

Product name

CXCL2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human CXCL2/MIP-2.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CXCL2 in Lane A: Daudi Whole Cell Lysate (30 µg). Samples were probed using a CXCL2 Polyclonal Antibody (Product # PA5-80529) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:11 kDa. Observed band size:11 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using CXCL2 Polyclonal Antibody (Product # PA5-80529) at 1:500 dilution. Lane A: Daudi Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 11 kDa. Observed band size: 11 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-CXCL2 Polyclonal Antibody (Product # PA5-80529) and a 7.9 kDa band corresponding to C-X-C motif chemokine 2 was observed LPS treated and secretion blocked HUVEC cells. Whole cell extracts (40 µg lysate) of HUVEC (Lane 1), PTI treated HUVEC (Lane 2), LPS treated HUVEC (Lane 3), LPS and PTI treated HUVEC (Lane 4) were electrophoresed using Novex™ 16% Tricine Protein Gel (Product # EC6695BOX). Resolved proteins were then transferred onto a PVDF membrane (Product # LC2002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:10000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Uncharacterised bands (*) at ~36 kDa and ~90 kDa was also detected across the panel.