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Learn500-P104-1MG
antibody from Invitrogen Antibodies
Targeting: CXCL2
CINC-2a, GRO2, GROb, MGSA-b, MIP-2a, SCYB2
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- ELISA [1]
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- Product number
- 500-P104-1MG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CXCL2 Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: APLATELRCQ CLQTLQGIHL KNIQSVKVKS PGPHCAQTEV IATLKNGQKA CLNPASPMVK KIIEKMLKNG KSN. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human GRO-beta (CXCL2). Anti-Human GRO-beta (CXCL2)-specific antibody was purified by affinity chromatography employing an immobilized Human GRO-beta (CXCL2) matrix. Sandwich ELISA: To detect Human GRO-beta (CXCL2) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human GRO-beta (CXCL2) (500-P104Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human GRO-beta (CXCL2). Western Blot: To detect Human GRO-beta (CXCL2) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human GRO-beta (CXCL2) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 1 mg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references The Global Phosphorylation Landscape of SARS-CoV-2 Infection.
NAP-2 Secreted by Human NK Cells Can Stimulate Mesenchymal Stem/Stromal Cell Recruitment.
Bouhaddou M, Memon D, Meyer B, White KM, Rezelj VV, Correa Marrero M, Polacco BJ, Melnyk JE, Ulferts S, Kaake RM, Batra J, Richards AL, Stevenson E, Gordon DE, Rojc A, Obernier K, Fabius JM, Soucheray M, Miorin L, Moreno E, Koh C, Tran QD, Hardy A, Robinot R, Vallet T, Nilsson-Payant BE, Hernandez-Armenta C, Dunham A, Weigang S, Knerr J, Modak M, Quintero D, Zhou Y, Dugourd A, Valdeolivas A, Patil T, Li Q, Hüttenhain R, Cakir M, Muralidharan M, Kim M, Jang G, Tutuncuoglu B, Hiatt J, Guo JZ, Xu J, Bouhaddou S, Mathy CJP, Gaulton A, Manners EJ, Félix E, Shi Y, Goff M, Lim JK, McBride T, O'Neal MC, Cai Y, Chang JCJ, Broadhurst DJ, Klippsten S, De Wit E, Leach AR, Kortemme T, Shoichet B, Ott M, Saez-Rodriguez J, tenOever BR, Mullins RD, Fischer ER, Kochs G, Grosse R, García-Sastre A, Vignuzzi M, Johnson JR, Shokat KM, Swaney DL, Beltrao P, Krogan NJ
Cell 2020 Aug 6;182(3):685-712.e19
Cell 2020 Aug 6;182(3):685-712.e19
NAP-2 Secreted by Human NK Cells Can Stimulate Mesenchymal Stem/Stromal Cell Recruitment.
Almeida CR, Caires HR, Vasconcelos DP, Barbosa MA
Stem cell reports 2016 Apr 12;6(4):466-473
Stem cell reports 2016 Apr 12;6(4):466-473
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Sandwich ELISA: To detect Human GRO-beta (CXCL2) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of CXCL2 Polyclonal Antibody (Product # 500-P104-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech CXCL2 Polyclonal Antibody, Biotin (Product # 500-P104BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human GRO-beta (CXCL2).
