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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- PA1-836-A488 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SNX1 Polyclonal Antibody, Alexa Fluor™ 488
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Green dye
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 1.0 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SNX-1 (green) in HeLa cells. The cells were fixed with 4% Paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a SNX-1 rabbit polyclonal antibody (Product # PA1-836-A488) at a concentration of 5 µg/mL in blocking buffer for 1 hour at room temperature. F-actin (red) was stained by Dylight 554 Phalloidin (Product #21834) and nuclei (blue) were stained with Hoechst 33342 (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
- Conjugate
- Green dye
