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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-46928 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- betacellulin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of betacellulin in MCF‚7 human breast cancer cell line and OVCAR‚3 human ovarian carcinoma cell line. Samples were incubated in betacellulin polyclonal antibody (Product # PA5-46928) using a dilution of 2 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for Betacellulin/BTC at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of betacellulin in immersion fixed paraffin-embedded sections of human lung cancer tissue. Samples were incubated in betacellulin polyclonal antibody (Product # PA5-46928) using a dilution of 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of betacellulin in immersion fixed paraffin-embedded sections of human pancreas. Samples were incubated in betacellulin polyclonal antibody (Product # PA5-46928) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to islets.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of betacellulin in Balb/3T3 mouse embryonic fibroblast cell line. Samples were incubated in betacellulin polyclonal antibody (Product # PA5-46928). Recombinant Human Betacellulin/BTC stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Betacellulin/BTC (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Betacellulin/BTC Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.04‚0.08 µg/mL.
