H00010535-M01

antibody from Abnova Corporation

Targeting: RNASEH2A AGS4, RNASEHI, RNHIA, RNHL

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00010535-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00010535-M01, RRID:AB_425852

Product name

RNASEH2A monoclonal antibody (M01), clone 3G5-F5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant RNASEH2A.

Antigen sequence

MDLSELERDNTGRCRLSSPVPAVCRKEPCVLGVDE
AGRGPVLGPMVYAICYCPLPRLADLEALKVADSKT
LLESERERLFAKMEDTDFVGWALDVLSPNLISTSM
LGRVKYNLNSLSHDTATGLIQYALDQGVNVTQVFV
DTVGMPETYQARLQQSFPGIEVTVKAKADALYPVV
SAASICAKVARDQAVKKWQFVEKLQDLDTDYGSGY
PNDPKTKAWLKEHVEPVFGFPQFVRFSWRTAQTIL
EKEAEDVIWEDSASENQEGLRKITSYFLNEGSQAR
PRSSHRYFLERGLESATSL


Isotype

IgG

Antibody clone number

3G5-F5

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

RNASEH2A monoclonal antibody (M01), clone 3G5-F5 Western Blot analysis of RNASEH2A expression in Jurkat ( Cat # L017V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of RNASEH2A expression in transfected 293T cell line by RNASEH2A monoclonal antibody (M01), clone 3G5-F5.Lane 1: RNASEH2A transfected lysate(33.4 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged RNASEH2A is 1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol