Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-52282 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RBM47 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: HAMNNLNGTE LEGSCLEVTL AKPVDKEQYS RYQKAARGGG AAEAAQQPSY VYSCDPYTLA YYGYPYNALI GPNRDYFVKA GSIRGRGRGA AGNRAPGPRG SYLGGYSAGR GIYSRYHEGK GKQQEKGYEL VPNLEIPTVN Highest antigen sequence identity to the following orthologs: Mouse - 94%, Rat - 94%.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RBM47 in human cell line BEWO using a RBM47 Polyclonal Antibody (Product # PA5-52282).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RBM47 in mouse cell line NIH-3T3 and rat cell line NBT-II using a RBM47 Polyclonal Antibody (Product # PA5-52282).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RBM47 in human cell lines A-549 and SK-MEL-30 using a RBM47 Polyclonal Antibody (Product # PA5-52282). Corresponding RBM47 RNA-seq data are presented for the same cell lines. Loading control: Anti-PARP1.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of RBM47 in human cell line A549 shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using a RBM47 Polyclonal Antibody (Product # PA5-52282).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RBM47 in human stomach tissue shows strong nuclear positivity in glandular cells. Samples were probed using a RBM47 Polyclonal Antibody (Product # PA5-52282).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RBM47 in human stomach and endometrium tissues using RBM47 Polyclonal Antibody (Product # PA5-52282). Corresponding RBM47 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RBM47 in human endometrium using RBM47 Polyclonal Antibody (Product # PA5-52282) shows low expression as expected.