Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [6]
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Validation data
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- Product number
- NBP1-83664 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-83664, RRID:AB_11029156
- Product name
- Rabbit Polyclonal RBM47 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, rat RBM47 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Loss of the multifunctional RNA-binding protein RBM47 as a source of selectable metastatic traits in breast cancer.
Vanharanta S, Marney CB, Shu W, Valiente M, Zou Y, Mele A, Darnell RB, Massagué J
eLife 2014 Jun 4;3
eLife 2014 Jun 4;3
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RBM47 Antibody [NBP1-83664] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RBM47 Antibody [NBP1-83664] - Analysis in human cell line BEWO.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RBM47 Antibody [NBP1-83664] - Analysis in human cell lines A-549 and SK-MEL-30 using Anti-RBM47 antibody. Corresponding RBM47 RNA-seq data are presented for the same cell lines. Loading control: Anti-PARP1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RBM47 Antibody [NBP1-83664] - Staining of human stomach shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RBM47 Antibody [NBP1-83664] - Staining of human endometrium shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RBM47 Antibody [NBP1-83664] - Staining of human fallopian tube shows moderate nuclear positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RBM47 Antibody [NBP1-83664] - Staining of human kidney shows moderate nuclear positivity in cells in tubules.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RBM47 Antibody [NBP1-83664] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RBM47 Antibody [NBP1-83664] - Staining in human colon and skeletal muscle tissues using NBP1-83664 antibody. Corresponding RBM47 RNA-seq data are presented for the same tissues.