MA5-45701

antibody from Invitrogen Antibodies

Targeting: KCNK3 K2p3.1, TASK, TASK-1, TASK1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

MA5-45701

Provider

Invitrogen Antibodies

Product name

KCNK3 Monoclonal Antibody (N374/48), APC

Antibody type

Monoclonal

Antigen

Other

Description

Mouse: 96% identity (156/161 amino acids identical). Human: 76% identity (163/161 amino acids identical).

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

N374/48

Vial size

100 µg

Concentration

1 mg/mL

Storage

4°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Rat Brain Membrane showing detection of ~50 kDa TASK1 Potassium Channel protein. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain membrane. Load: 15 µg. Blocking: 2% BSA and 2% Skim Milk in 1X TBST. Samples were incubated with TASK1 Potassium Channel monoclonal antibody (Product # MA5-45701) at 1:1,000 for 16 hours at 4°C, followed by Goat Anti-Mouse IgG: HRP at 1:2,000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~50 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with TASK1 Potassium Channel monoclonal antibody (Product # MA5-45701) at 1:50 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TASK1 Potassium Channel antibody (D) Composite.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with TASK1 Potassium Channel monoclonal antibody (Product # MA5-45701) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min RT, 5 min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) TASK1 Potassium Channel antibody. (D) Composite.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with TASK1 Potassium Channel monoclonal antibody (Product # MA5-45701) at 1:50 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TASK1 Potassium Channel antibody (D) Composite.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with TASK1 Potassium Channel monoclonal antibody (Product # MA5-45701) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min RT, 5 min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) TASK1 Potassium Channel antibody. (D) Composite.