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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Other assay [1]
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- Product number
- A302-040A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- G3BP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human G3BP2 by western blot. Samples: Whole cell lysate (10 µg) from HeLa, MCF-7, Jurkat, Hep-G2, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G3BP2 antibody (Product # A302-040A lot 4) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human G3BP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-G3BP2 antibody A302-040A (lot A302-040A-3) used for IP at 3 µg per reaction. G3BP2 was also immunoprecipitated by rabbit anti-G3BP2 antibody A302-041A. For blotting immunoprecipitated G3BP2, A302-040A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human G3BP2 by western blot. Samples: Whole cell lysate (15 µg) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G3BP2 antibody A302-040A (lot A302-040A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human G3BP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-G3BP2 antibody (Product # A302-040A; Lot 4) used for IP at 6 µg per reaction. G3BP2 was also immunoprecipitated by a previous lot of this antibody (Product # A302-040A; Lot 3) and rabbit anti-G3BP2 antibody (Product # A302-041A). For blotting immunoprecipitated G3BP2, Product # A302-040A was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 1 seconds.
