NBP2-14147

antibody from Novus Biologicals

Targeting: KCTD13 FKSG86, PDIP1, POLDIP1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-14147

Provider

Novus Biologicals

Product name

Rabbit Polyclonal POLDIP1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human POLDIP1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: POLDIP1 Antibody [NBP2-14147] - Analysis in control (vector only transfected HEK293T lysate) and KCTD13 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: POLDIP1 Antibody [NBP2-14147] - Staining of human pancreas shows low expression as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: POLDIP1 Antibody [NBP2-14147] - Staining of human testis shows high expression.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: POLDIP1 Antibody [NBP2-14147] - Staining of human prostate shows moderate to strong nuclear positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: POLDIP1 Antibody [NBP2-14147] - Staining of human testis shows moderate to strong nuclear positivity in Leydig cells and cells in seminiferous ducts.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: POLDIP1 Antibody [NBP2-14147] - Staining of human small intestine shows moderate to strong nuclear positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: POLDIP1 Antibody [NBP2-14147] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: POLDIP1 Antibody [NBP2-14147] - Staining of human skeletal muscle shows very weak positivity in myocytes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: POLDIP1 Antibody [NBP2-14147] - Staining in human testis and skeletal muscle tissues.. Corresponding KCTD13 RNA-seq data are presented for the same tissues.