LS-C154233

antibody from LSBio

Targeting: EIF3E eIF3-p48, EIF3S6, INT6

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

LS-C154233

Provider

LSBio

Product name

EIF3E Antibody (C-Terminus) LS-C154233

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse, Rat, Bovine, Canine, Simian

Host

Rabbit

Isotype

IgG

Storage

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-eIF3S6/Int6 Antibody - Western Blot. Western blot of affinity purified anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6 in whole cell extracts from murine (HC-11 and NIH3T3), monkey (CV-1 and Cos-1), and human (HEK293T) cell lines as well as over-expressed eIF3S6/Int6 (control transfected flag-tagged Int6). The identity of the higher and lower molecular weight bands is unknown. The band at ~48 kD, indicated by the arrowhead, corresponds to flag-tagged EIF3S6/Int6. Primary antibody was used at 1:1000. Personal communication, J. Lee, NCI, Bethesda, MD.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-eIF3S6/Int6 Antibody - Western Blot. Western blot of affinity purified anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6. Specific staining is not present in lysates containing lentiviral knockdown vectors (shRNA #1 and #2. Control vectors, specifically a scrambled sequence (Ctl NS) and a sequence against an unrelated gene (Ctl #2), were also used. Personal communication, J. Lee, NCI, Bethesda, MD.