LS-C745313

antibody from LSBio

Targeting: EIF3E eIF3-p48, EIF3S6, INT6

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

LS-C745313

Provider

LSBio

Product name

EIF3E Antibody (C-Terminus) LS-C745313

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse, Rat, Bovine, Canine, Simian

Host

Rabbit

Isotype

IgG

Storage

Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot using the affinity purified anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6 in whole cell extracts from murine (HC-11 and NIH3T3), monkey (CV-1 and Cos-1), and human (HEK293T) cell lines as well as over-expressed eIF3S6/Int6 (control transfected flag-tagged Int6).  The identity of the higher and lower molecular weight bands is unknown.  The band at ~48 kDa, indicated by the arrowhead, corresponds to flag-tagged EIF3S6/Int6.  Primary antibody was used at 1:1000.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot using the affinity purified anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6. Specific staining is not present in lysates containing lentiviral knockdown vectors (shRNA #1 and #2). Control vectors, specifically a scrambled sequence (Ctl NS) and a sequence against an unrelated gene (Ctl #2), were also used.