Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- PAB10203 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10203, RRID:AB_1674724
- Product name
- Eif3e polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of Eif3e.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Int6 expression can predict survival in early-stage non-small cell lung cancer patients.
A new common integration site, Int7, for the mouse mammary tumor virus in mouse mammary tumors identifies a gene whose product has furin-like and thrombospondin-like sequences.
Fission yeast Int6 is not essential for global translation initiation, but deletion of int6(+) causes hypersensitivity to caffeine and affects spore formation.
Buttitta F, Martella C, Barassi F, Felicioni L, Salvatore S, Rosini S, D'Antuono T, Chella A, Mucilli F, Sacco R, Mezzetti A, Cuccurullo F, Callahan R, Marchetti A
Clinical cancer research : an official journal of the American Association for Cancer Research 2005 May 1;11(9):3198-204
Clinical cancer research : an official journal of the American Association for Cancer Research 2005 May 1;11(9):3198-204
A new common integration site, Int7, for the mouse mammary tumor virus in mouse mammary tumors identifies a gene whose product has furin-like and thrombospondin-like sequences.
Lowther W, Wiley K, Smith GH, Callahan R
Journal of virology 2005 Aug;79(15):10093-6
Journal of virology 2005 Aug;79(15):10093-6
Fission yeast Int6 is not essential for global translation initiation, but deletion of int6(+) causes hypersensitivity to caffeine and affects spore formation.
Bandyopadhyay A, Matsumoto T, Maitra U
Molecular biology of the cell 2000 Nov;11(11):4005-18
Molecular biology of the cell 2000 Nov;11(11):4005-18
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using Eif3e polyclonal antibody (Cat # PAB10203) shows detection of endogenous Eif3e. Specific staining is not present in lysates containing lentiviral knockdown vectors (shRNA #1 and #2. Control vectors, specifically a scrambled sequence (Ctl NS) and a sequence against an unrelated gene (Ctl #2), were also used.Personal communication, J.Lee, NCI, Bethesda, MD.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using Eif3e polyclonal antibody (Cat # PAB10203) shows detection of endogenous Eif3e in whole cell extracts from murine (HC-11 and NIH/3T3), monkey (CV-1 and COS-1), and human (HEK293T) cell lines as well as over-expressed Eif3e (control transfected flag-tagged Eif3e). The identity of the higher and lower molecular weight bands is unknown. The band at ~48 KDa, indicated by the arrowhead, corresponds to flag-tagged Eif3e.Primary antibody was used at 1:1000. Personal communication, J.Lee, NCI, Bethesda, MD.