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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Flow cytometry [2]
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- Product number
- RQ6619 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- EIF3E Antibody / eIF-3 p48
- Antibody type
- Polyclonal
- Description
- This highly specific EIF3E antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry/Direct ELISA applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the EIF3E antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human K562, 2) human Raji and 3) mouse ANA-1 cell lysate with EIF3E antibody. Expected molecular weight: 48-52 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human appendiceal adenocarcinoma tissue with EIF3E antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human placental tissue with EIF3E antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human spleen tissue with EIF3E antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human HeLa cells with EIF3E antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= EIF3E antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of mouse RAW264.7 cells with EIF3E antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= EIF3E antibody.
