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Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [4]
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- Product number
- NB100-64988 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-64988, RRID:AB_2075680
- Product name
- Mouse Monoclonal CD4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. NB100-64988 recognizes the rat CD4 cell surface glycoprotein, a 55kD molecule expressed by helper T cells and weakly by monocytes. This antibody inhibits proliferation and IL-2 production in the MLR reaction. *This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde) (3). This product is routinely tested in flow cytometry on rat splenocytes.
- Reactivity
- Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.25 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Effect of acute stress on immune cell counts and the expression of tight junction proteins in the duodenal mucosa of rats.
Inhibition of retromer activity by herpesvirus saimiri tip leads to CD4 downregulation and efficient T cell transformation.
Lee HS, Kim DK, Kim YB, Lee KJ
Gut and liver 2013 Mar;7(2):190-6
Gut and liver 2013 Mar;7(2):190-6
Inhibition of retromer activity by herpesvirus saimiri tip leads to CD4 downregulation and efficient T cell transformation.
Kingston D, Chang H, Ensser A, Lee HR, Lee J, Lee SH, Jung JU, Cho NH
Journal of virology 2011 Oct;85(20):10627-38
Journal of virology 2011 Oct;85(20):10627-38
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: CD4 Antibody (W3/25) [NB100-64988] - Staining of frozen rat spleen with Mouse anti Rat CD4.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD4 Antibody (W3/25) [NB100-64988] - Staining of stimulated rat spleen cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Cell Surface): CD4 Antibody (W3/25) [NB100-64988] - Rat Splenocytes were stained with NB100-64988 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. The antibodies were directly conjugated to Alex Fluor 488.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD4 Antibody (W3/25) [NB100-64988] - Analysis using the Biotin conjugate of NB100-64988 (NB100-64988B), rat splenocytes were stained.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD4 Antibody (W3/25) [NB100-64988] - Detection of CD4 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with (A) Mouse Anti-Rat CD4 Monoclonal Antibody (Catalog # NB100-64988) or (B) Mouse IgG2A Isotype Control Antibody (Catalog # MAB003) followed by anti-Mouse IgG APC-conjugated secondary antibody (Catalog # F0101B) and Mouse Anti-Rat CD3 epsilon PE conjugated Monoclonal Antibody.
