MA1-10800

antibody from Invitrogen Antibodies

Targeting: CD4

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-10800

Provider

Invitrogen Antibodies

Product name

CD4 Monoclonal Antibody (10C12)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

MA1-10800 detects CD4 Cytoplasmic Tail from human samples. MA1-10800 has been successfully used in ELISA, immunofluorescence, and Western blot applications. The MA1-10800 immunogen is a synthetic peptide corresponding to residues L S E K K T C Q C P H R F Q K T C S P I of human CD4.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

10C12

Vial size

100 µg

Concentration

0.9 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

p65/miR-23a/CCL22 axis regulated regulatory T cells recruitment in hepatitis B virus positive hepatocellular carcinoma.

Li ZQ, Wang HY, Zeng QL, Yan JY, Hu YS, Li H, Yu ZJ
Cancer medicine 2020 Jan;9(2):711-723

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Lower miR-23a expression was correlated with higher level of CCL22 expression and intratumoral Treg recruitment in HBV-positive HCC. A, HBV infection was associated with patient survival rate of HCC. Patients carrying HBV (n = 30) had significantly poorer prognosis than HBV - patients (n = 30). B, HBV - tissues (n = 30) and HBV + tissues (n = 30) expressed significantly lower level of miR-23a. C, HBV - tissues and HBV + tissues expressed significantly higher mRNA level of CCL22. D, HBV - tissues and HBV + tissues expressed significantly higher mRNA level of Foxp3. E, The protein levels of CCL22, Foxp3, p-p65, and p65 in normal control, HBV - and HBV + tumor tissues were evaluated by western blotting. F, The gray scale analysis of CCL22, Foxp3, p-p65, and p65 in normal control, HBV- and HBV+ tumor tissues. In ascending order: normal < HBV - < HBV + . G, Foxp3 signals (red) were colocalized with CD4 (green) signals in tissues. The ratios of Foxp3 + CD4 + cells were gradually increased from normal, HBV - HCC to HBV + HCC tissues. H. MiR-23a level was inversely correlated with CCL22 expression in HCC tissues, but not in normal control. I. MiR-23a level was inversely correlated with Foxp3 expression in HCC tissues, but not in normal control. Error bars represented mean +- SD. ** P < .01 and * P < .05. HBV + , HCC tissues with HBV infection. HBV - , HCC tissues without HBV infection. Normal, normal liver samples